Bacteriologic Study on Conjunctiva of Eyeball Donor and Antibiotic Sensitivity Test.
- Author:
Tae Won HAHN
1
;
Yeun Mi SHIN
;
Jae Ho KIM
Author Information
1. Department of Ophthalmology, College of Medicine, Catholic University of Korea, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
Antibiotic sensitivity;
Bacterial flora;
Eyeball donor
- MeSH:
Agar;
Bacteria;
Coagulase;
Conjunctiva*;
Cornea;
Diffusion;
Drug Resistance, Microbial;
Fungi;
Gram-Negative Bacteria;
Gram-Positive Bacteria;
Humans;
Pseudomonas aeruginosa;
Staphylococcus;
Staphylococcus aureus;
Tissue Donors*
- From:Journal of the Korean Ophthalmological Society
1998;39(2):270-276
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
We evaluated the distribution of bacterial flora in conjunctiva of eyeball donor and antibiotic sensitivity of the cultured bacteria. Specimens were obtained from inferior conjunctiva cul de sac with sterile cotton-tipped applicator between 1-5 hours after death and plated on routine agar plates. Antibiotic sensitivity test was done by disk diffusion method. From 1994 to 1995, a total number of 338 specimens were collected from donor eyes. Among them, 191(56.5%) were culture positive and 147(43.5%) were culture negative. Among cultured 125 signle organisms, 86 organisms(68.8%) were Gram positive bacteria, 37(29.6%) Gram negative bacteria and 2(1.6%) fungi. Coagulase Negative Staphylococcus (CNS) (29.2%) and Pseudomonas aeruginosa (10.9%) were the most commonly isolated microorganisms. Antibiotic sensitivity test demonstrated a high rate of resistance in common pathogenic microorganisms such as CNS, Staphylococcus aureus and Pseudomonas aeruginosa. These microorganisms showed antibiotic resistance even to fluoroquinolone. Thus, aseptic enucleation and proper antibacterial treatment are prerequisite for the preparation of the donor cornea.
