Mobilization and Collection of the Peripheral Blood Stem Cell from Normal Bone Marrow by G-CSF Alone.
- Author:
Mi Hyun TAE
1
;
Dong Jin IM
;
Ki Woong SUNG
;
Hong Hoe KOO
;
Dae Won KIM
;
Hyung Rok KIM
Author Information
1. Department of Pediatrics, Samsung Seoul Hospital, Sungkyunkwan University School of Medicine, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
Peripheral blood stem cell transplantation;
Mobilization;
G-CSF;
Brain tumor
- MeSH:
Autografts;
Blood Platelets;
Bone Marrow Transplantation;
Bone Marrow*;
Brain Neoplasms;
Drug Therapy;
Feasibility Studies;
Granulocyte Colony-Stimulating Factor*;
Humans;
Leukapheresis;
Neutrophils;
Peripheral Blood Stem Cell Transplantation;
Radiotherapy;
Stem Cells*;
Tissue Donors;
Transplantation, Autologous;
Transplantation, Homologous
- From:Korean Journal of Pediatric Hematology-Oncology
2000;7(2):233-241
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: Peripheral blood stem cells (PBSC) can be mobilized by use of G-CSF alone from normal bone marrow. In this study, feasibility of mobilization, collection, and hematologic recovery after transplantion were evaluated. METHPDS: From normal undamaged bone marrow of normal PBSC donors and patients with high risk brain tumor who had no experience of chemotherapy or radiotherapy, PBSC was mobilized by use of G-CSF alone. Ten ug/kg/day of G-CSF was injected subcutaneously and leukaphereses were done on the fourth and fifth day of G-CSF injection. Nucleated cells (NC), mononuclear cells (MNC), CD34+ cells and colony forming cells (CFC) were counted. Hematologic recovery was evaluated in 4 autologous transplantations and 6 allogeneic transplantations, 4 of which were done after T cell depletion. RESULTS: Twenty four leukaphereses were done in 6 normal donors and 6 patients with high risk brain tumor. Median 603.3 (342.6~834.5) mL/kg of blood was processed for median 447 (392~549) minutes. Collected cells were as follows; NC: 11.88 (3.11~25.89)x108/kg of donor, MNC: 8.66 (2.61~12.84)x10(8)/kg of donor, CD34+ cells: 7.05 (2.95~11.73)x10(6)/kg of donor, CFC: 25.38 (3.62~35.27)x10(5)/kg of donor, respectively. In allogeneic transplantation, time to reach absolute neutrophil count (ANC)> 500/uL, 1,000/ uL and platelet count> 20,000/uL, 50,000/uL were 10 (9~15) days, 11 (9~16) days, 11 (8~30) days, and 32 (19~156) days, respectively. In autologous transplantation, time to reach ANC> 500/uL, 1,000/uL and platelet count> 20,000/uL, 50,000/uL were 9.5 (9~11) days, 10.0 (9~11) days, 10.5 (9~13) days, and 14.5 (13~17) days, respectively. CONCLUSION: Sufficient number of undamaged PBSC was collected from normal bone marrow by use of G-CSF alone. Hematologic recovery after transplantaion was more rapid than allogeneic bone marrow transplantation or autologous PBSCT which was done with cells collected after chemotherapy and/or radiotherapy.
