Effects of Benzoapyrene, 2-Bromopropane, Phenol and 2,3,7,8-Tetrachlorodibenzo-p-Dioxin on Proinflammatory Cytokines Gene Expression by Mice Spleen Cells.
- Author:
Ho Jun KIM
1
;
Bit Na KANG
;
Sung Whan CHO
;
Hwa Young SON
;
Kyu Shik JEONG
;
Sang Joon PARK
;
Sung Ho KIM
;
Se Ra KIM
;
Tae Hwan KIM
;
Mi Young AN
;
Si Yun RYU
Author Information
1. College of Veterinary Medicine, Chungnam National University, Daejeon 305-764, Korea. syryu@cnu.ac.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
cytokine;
TCDD;
benzo[a]pyrene;
2-bromopropane;
phenol
- MeSH:
Animals;
Antigens, CD3/immunology;
Apoptosis/drug effects;
Benzo(a)pyrene/*toxicity;
Cells, Cultured;
Cytokines/*biosynthesis/genetics;
Environmental Pollutants/*toxicity;
Gene Expression/drug effects;
Hydrocarbons, Brominated/*toxicity;
Male;
Mice;
Mice, Inbred C3H;
Phenol/*toxicity;
RNA/chemistry/genetics;
Reverse Transcriptase Polymerase Chain Reaction;
Spleen/*drug effects/metabolism;
Tetrachlorodibenzodioxin/*toxicity
- From:Journal of Veterinary Science
2002;3(4):247-254
- CountryRepublic of Korea
- Language:English
-
Abstract:
The detrimental effects of environmental pollutants on the health of the individual are generally accepted, although the mechanisms of these effects remain to be incompletely understood. In the present study, we examined the effects of B[a]P, 2-BP, phenol and TCDD on proinflammatory cytokine gene expression in mice spleen cells which were stimulated with anti-CD3. 10-9M TCDD increased IFN gammar and TNF alpha gene expression, but suppressed IL-1 gene expression. 10-6M phenol inhibited IL-1, IL-6 and TNF alpha gene expression, and 10-6M of 2-BP downregulated TNF alpha gene expression. However, 10-6M of B[a]P did not influence on IL-1, IL-6, IFN gammar and TNF alpha gene expression. These findings suggest that TCDD may impair the immune functions of mice by enhancing proinflammatory cytokines production, whereas phenol and 2-BP may impair the functions by inhibiting the production of these cytokines.
