Genetic Polymorphism of the Serum Proteins of Horses in Jeju.
- Author:
Jin Ah SHIN
1
;
Young Hoon YANG
;
Hee Seok KIM
;
Young Min YUN
;
Kyoung Kap LEE
Author Information
1. Department of Veterinary Medicine, Agriculture & Life Sciences, Cheju National University, Jeju, Korea. leekk@cheju.ac.kr
- Publication Type:Original Article
- Keywords:
serum protein;
polymorphism;
phenotype;
frequency;
heterozygosity;
HPAGE;
Cheju native horse
- MeSH:
Alleles;
Animals;
Blood Proteins/*genetics;
Electrophoresis, Polyacrylamide Gel;
Esterases/genetics;
Genetic Variation;
Horses/blood/*genetics;
Polymorphism, Genetic;
Serum Albumin/genetics;
Transferrin/genetics;
Vitamin D-Binding Protein/genetics
- From:Journal of Veterinary Science
2002;3(4):255-263
- CountryRepublic of Korea
- Language:English
-
Abstract:
The study was carried out to investigate the genetic polymorphism of the serum proteins of horses in Cheju. They were assigned to three groups; 45 Cheju native horses(CNH), 60 Cheju racing horses(CRH) and 60 Thoroughbreds(TB). We analyzed the phenotypes and gene frequencies of serum proteins which were albumin (Alb), vitamin-D binding protein(GC), esterase (ES), A1B glycoprotein(A1B) and transferrin(TF) loci using horizontal polyacrylamide gel electrophoresis (HPAGE).All of the loci, except A1B in TB, showed polymorphisms and different allelic and phenotypic frequencies in all three groups. ESS and TFF1 were not observed in CNH. Allelic frequencies of AlbB, ESI, TFD and TFF1 were high in TB. All of the loci, except ES locus in CRH, appeared to be in a state of Hardy-Weinberg equilibrium from goodness-of-fit test in all three groups Heterozygosity estimates at Alb, ES and TF loci were high, but GC and A1B loci were low in all three groups. Average heterozygosities in CNH, CRH and TB were 0.3535, 0.3555 and 0.2726, respectively. Results showed differences in the frequencies of alleles and phenotypes of several serum protein loci between CNH and CRH, suggested that CRH might be crossed with other breeds of horses in some degree.
