The Apoptotic Molecular Changes of Cellular Injury in Mouse Testis Induced by Endocrine Disrupting Chemicals.
- Author:
Eun Hui WANG
1
;
Kweon Heang LEE
;
Ki Hwa YANG
;
Jinsuk LEE
;
Eun Sun JUNG
;
Chang Suk KANG
;
Yeong Jin CHOI
Author Information
1. Department of Clinical Pathology, The Catholic University of Korea, Seoul 150-713, Korea. mdyjchoi@catholic.ac.kr
- Publication Type:Original Article
- Keywords:
Bisphenol A;
2-Bromopropane;
Diethylstilbesterol;
Spermatogenesis;
Testis
- MeSH:
Animals;
Apoptosis;
Caspase 1;
Cell Proliferation;
Endocrine Disruptors*;
Fas Ligand Protein;
Germ Cells;
Mice*;
Mice, Inbred ICR;
Proliferating Cell Nuclear Antigen;
Ribonucleases;
Spermatogenesis;
Testis*
- From:Korean Journal of Pathology
2004;38(4):228-237
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Spermatogenesis is regulated by various cellular reactions, and especially cell proliferation and apoptosis. METHODS: We investigated the morphological changes and the apoptotic molecular changes in mouse testis induced by the endocrine disrupting chemicals. ICR mice were treated with bisphenol A (BPA), 2-bromopropane (2-BP) and diethylstilbesterol (DES). Histological examination and immunohistochemical staining, TUNNEL staining and RNAse protection assay were conducted. RESULTS: Testes treated with BPA showed normal spermatogenesis and the proliferation activity, and the density of the cells was similar with those in the control. 2-BP and DES groups, which showed a decrease of germ cells near the basal layer and degenerative changes. The proliferative activity identified by PCNA staining was significantly decreased in the 2-BP and DES groups (p<0.05). The apoptosis was significantly increased in the 2-BP group however, a significant decrease was noted in the BPA group (p<0.05). Among apoptosis-related molecules, the expression of Fas, Fas ligand, TRAIL, TNFp55 and caspase 1, 3, 6 and 8 were changed according to the change of the degree of apoptosis in all groups. CONCLUSIONS: Endocrine disrupting chemicals induced cellular injury in mouse testis through the changes of proliferative activity and apoptosis which was regulated by a number of apoptosis-related molecules. This probably results in the abnormality of spermatogenesis in mouse testis.
