Development and evaluation of a competitive enzyme-linked immunosorbent assay using a monoclonal antibody for diagnosis of severe fever with thrombocytopenia syndrome virus in bovine sera.
10.4142/jvs.2016.17.3.307
- Author:
Hyojin LEE
1
;
Eun Ju KIM
;
Jae Young SONG
;
Jeong Soo CHOI
;
Ji Youn LEE
;
In Soo CHO
;
Yeun Kyung SHIN
Author Information
1. Division of Viral Disease, Animal and Plant Quarantine Agency, Anyang 14086, Korea. shinyk2009@korea.kr
- Publication Type:Original Article
- Keywords:
cattle;
enzyme-linked immunosorbent assay;
immunofluorescence assay;
severe fever with thrombocytopenia syndrome
- MeSH:
Animals;
Antibodies;
Antibodies, Monoclonal;
Antiviral Agents;
Blood Cell Count;
Bunyaviridae;
Cattle;
Cattle Diseases;
China;
Communicable Diseases;
Diagnosis*;
Enzyme-Linked Immunosorbent Assay*;
Fever*;
Fluorescent Antibody Technique;
Humans;
Immune Sera;
Nucleoproteins;
Phlebovirus;
Sensitivity and Specificity;
Thrombocytopenia*;
Vaccines
- From:Journal of Veterinary Science
2016;17(3):307-314
- CountryRepublic of Korea
- Language:English
-
Abstract:
Severe fever with thrombocytopenia syndrome (SFTS) caused by the SFTS virus (SFTSV), a phlebovirus in the family Bunyaviridae, is an emerging tick-borne infectious disease that impacts humans. This disease manifests as a decreased blood cell count and multi-organ failure, with a case-fatality rate of more than 12% in China. Because vaccines or antiviral drugs for the treatment of this disease are not available, monitoring the SFTS circulation in animals and controlling the tick-mammal cycle are important for preventing SFTS. Monoclonal antibodies against the recombinant nucleoprotein of SFTSV were generated to develop a competitive enzyme-linked immunosorbent assay (cELISA) for the detection of antibodies against SFTSV infection in cattle. The specificity and sensitivity of cELISA was assessed by comparing the results of this assay to those of an immunofluorescence assay (IFA). The results of the cELISA using 416 field bovine serum samples and laboratory-immunized positive sera showed 98.1% consistency with those of the IFA. The cELISA used in this study did not show cross-reactivity with antisera against other viral cattle diseases. The cELISA presented in this study can be applied to detect antibodies against SFTSV in cattle.
