The Effect of Vitamin E on the Composition of inflammatory Cells in Alveoli after Paraquat Intoxication in Rats.
10.4046/trd.1997.44.6.1332
- Author:
Kwang Seon SONG
1
;
Won Yeon LEE
;
Do Yeun CHO
;
Suk Joong YONG
;
Kye Chul SHIN
Author Information
1. Department of Internal Medicine, Yonsei Wonju College of Medicine, Wonju, Korea.
- Publication Type:Original Article
- Keywords:
Acute lung injury;
Paraquat;
Vitamin E;
Bronchoalveolar lavage
- MeSH:
Acute Lung Injury;
Animals;
Bronchoalveolar Lavage;
Cell Count;
Free Radicals;
Lipid Peroxidation;
Lung Injury;
Mortality;
Neutrophils;
Oxygen;
Paraquat*;
Pulmonary Fibrosis;
Rats*;
Therapeutic Irrigation;
Vitamin E*;
Vitamins*
- From:Tuberculosis and Respiratory Diseases
1997;44(6):1332-1342
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Acute pulmonary injury by paraquat are caused by multiple mechanisms including direct injury with oxygen free radicals and several mediators released from inflammatory cells. In order to clarify whether vitamin E could reduce tissue damages induced by intraperitoneal administaration of paraquat and to investigate the pathogenetic mechanisms of paraquat-induced pulmonary injury, vitamin E as a free radical scavenger was administered. METHODS: Rats were divided into three groups (group 1: control, group 2 : paraquat treated group, group 3 : paraquat and vitamin E treated group). Animals were sacrificed on day 1, day 2, day 3, and day 8 after the administration of saline, paraquat, or paraquat/vitamin E. RESULTS: Treatment with vitamin E decreased the death rate of rats treated with paraquat. Comparing with control group (1.37 x 106/ml), mean total cell counts recovered from the lavage fluid from animals treated with paraquat (1.65 x 106/ml) were increased(p=0.06). Magnitudes of increament of the total cell counts on the Day 8 in the vitamin E treated group were smaller than those of the animals treated with paraquat alone. The neutrophils began to appear in significant amounts in the lavage fluid on Day 8 after the administration of paraquat(37.0 + 12.7%). A significant decreasing neutrophil concentration at Day 8 was observed in the paraquat/vitamin E treated group(20.6 + 13.4 %). Histologically the degree of pulmonary fibrosis was most prominent in the paraquat treated group while diffuse alveolar damage was continuously observed in the paraquat/vitamin E treated group and extensive interstitial lymphocytic infiltration was seen in the paraquat/vitamin E treated group. The paraquat/vitamin E treated group showed the less histologic changes. CONCLUISON: In this study vitamin E acting as a scavenger of neutrophil-derived free radicals and suppressant of lipid peroxidation, seemed to be the effective antioxidant in the inhibition of paraquat-induced pulmonary injury.
