Relation of Interleukin-10 in Bronchoalveolar Lavage Fluid and Airway Inflammation in Bronchial Asthma.
- Author:
Sook Young LEE
1
;
Hung Gue YOUN
;
Youn SHIN
;
Sang Haak LEE
;
Seok Chan KIM
;
Kan Hyoung KIM
;
Hwa Sik MOON
;
Jeong Sup SONG
;
Sung Hak PARK
Author Information
- Publication Type:Original Article
- Keywords: bronchial asthma; interleukin-10; eosinophil
- MeSH: Asthma*; Biopsy; Bronchoalveolar Lavage Fluid*; Bronchoalveolar Lavage*; Cell Count; Cytokines; Enzyme-Linked Immunosorbent Assay; Eosinophils; Humans; Inflammation*; Interleukin-10*; Lung; Lymphocytes; Methacholine Chloride; Th2 Cells
- From:Tuberculosis and Respiratory Diseases 1999;46(1):44-52
- CountryRepublic of Korea
- Language:Korean
- Abstract: BACKGROUND: Airway infiltration by inflammatory cells, particularly of eosinophils, is one of the characteristic features of asthma. Several mechanisms for the recruitment of eosinophil is focused on the CD4+ T lymphocyte for the preferential production of Th2-derived cytokines. Interleukin-10(IL-10) is identified cytokine with potent antiinflammatory activity. This molecule has been shown to inhibit the release of cytokine from inflammatory cells including Th2 cell, and also to inhibit eosinophil survival. We therefore attempted to determine whether decreased synthesis of IL-10 in the lung of bronchial asthma may contribute to inflammation that is characteristics of this dease. METHOD: Subjects were patients with bronchial asthma(n=23) and normal controls(n=11). IL-10 produced from peripheral mononuclear cell(PBMC) and in bronchoalveolar lavage(BAL) fluid was measured by ELISA method. Degree of bronchial inflammation was assessed by total cell counts and eosinophil percents in BAL fluid, eosinophil infiltration on bronchial biopsy tissue and PC20 for methacholine. RESULTS: The IL-10 level produced by PBMC and in BAL fluid from patient with bronchial asthma were not different with normal controls(respectively, 901.6+/-220.4 pg/ml, 810.9+/-290.8pg/ml for PBMC, 24.5+/-9.5pg/ml, 30.5+/-13.5 pg/ml for BAL fluid p>0.05). There were significant negative correlation between IL-10 in BAL fluid and eosinophil percents in BAL fluid or degree of eosinophil infiltration in bronchial biopsy(respectively r=-0.522, r=-0.4486 p<0.05). However there was no difference of IL-10 level according to PC20 for methacholine. There were no correlation between IL-10 production by PBMC and peripheral blood eosinophil counts or serum eosinophilic cationic protein levels(respectively r=0.1146, r=0.0769 p>0.05). CONCLUSION: These observation suggest that IL-10 may participate but not acts the crucial role in regulation of the airway inflammation in bronchial asthma.
