rhBMP-2 using biphasic calcium phosphate block as a carrier induces new bone formation in a rat subcutaneous tissue.
10.5051/jkape.2008.38.Suppl.355
- Author:
Joon Il KIM
1
;
Jeong Ho YUN
;
Gyung Joon CHAE
;
Sung Won JUNG
;
Chang Sung KIM
;
Kyoo Sung CHO
Author Information
1. Department of Periodontology, Research Institute for Periodontal Regeneration, College of Dentistry, Yonsei University, Korea. kscho@yuhs.ac
- Publication Type:Original Article
- Keywords:
rhBMP-2;
biphasic calcium phosphate block;
carrier;
ectopic bone formation
- MeSH:
Animals;
Bone and Bones;
Bone Development;
Calcium;
Humans;
Hydroxyapatites;
Male;
Osteogenesis;
Rats;
Rats, Sprague-Dawley;
Subcutaneous Tissue
- From:The Journal of the Korean Academy of Periodontology
2008;38(Suppl):355-362
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: The carrier for the delivery of bone morphogenetic proteins(BMPs) should also serve as a scaffold for new bone growth. In addition, predictable bone formation in terms of the volume and shape should be guaranteed. This study evaluated the ectopic bone formation of recombinant human BMP-2(rhBMP-2) using a micro macroporous biphasic calcium phosphate (MBCP: mixture of betaTCP and HA) block as a carrier in a rat subcutaneous assay model. MATERIALS AND METHODS: Subcutaneous pockets were created on the back of 40 male Sprague-Dawley rats. In the pockets, rhBMP-2/MBCP and MBCP alone were implanted. The blocks were evaluated by histological and histometric parameters after a healing interval of 2 weeks (each 10 rats; MBCP and rhBMP-2/MBCP) or 8 weeks (each 10 rats; MBCP and rhBMP-2/MBCP). RESULTS: The shape and volume of the block was maintained stable over the healing period. No histological bone forming activity was observed in the MBCP alone sites after 2 weeks and there was minimal new bone formation at 8 weeks. In the rhBMP-2/MBCP sites, new bone formation was evident in the macropores of the block. The new bone area at 8 weeks was greater than at 2 weeks. There was a further increase in the quantity of new bone with the more advanced stage of remodeling. CONCLUSIONS: A MBCP block could serve as a carrier system for predictable bone tissue engineering using rhBMPs.
