Endotoxin Induced Uveitis Model.
- Author:
Min Ho KIM
1
;
Hae Suk KIM
;
Choun Ki JOO
Author Information
1. Department of Ophthalmology, Catholic University Medical College, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
Endotoxin;
ICAM-1;
lipopolysacharide;
LFA-1;
Uveitis
- MeSH:
Animals;
Ciliary Body;
Inflammation;
Intercellular Adhesion Molecule-1;
Iris;
Lymphocyte Function-Associated Antigen-1;
Rats;
Salmonella;
Uvea;
Uveitis*;
Uveitis, Anterior
- From:Journal of the Korean Ophthalmological Society
1997;38(6):969-975
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Endotoxin-induced uveitis model was produced in Lewis rats by footpad injection of Salmonella endotoxin(lipopolysaccharide, LPS). The clinical course and histological examination were observed at intervals of two hours. Immunohistochemical staining of intercellular adhesion molecule-1(ICAM-1) and lymphoyte function associated antigen-1(LFA-1) were also peformed. Initial intraocular inflammatory signs were observed at 8-10 hours after injection. Clinical and histological abnormalities peaked at 24-48 hours and were resolving after 72 hours. Histological changes were limited to anterior uvea. ICAM-1 expression was first noted on cells of iris and ciliary body and LFA-1 was expressed on infiltring inflammatory cells 8 hours after the injection and increased by 24 hours and disappeared after 72 hours. LPS induced uveitis in the rat provides a simple, reproducible model for anterior uveitis. ICAM-1 and LFA-1 expression in uveal tract are increased during early phase of uveitis and may enhance the adherence of inflammatory cells with the subsequent initiation of inflammation.
