Effect of radiation dosage changes on the cell viability and the apoptosis induction on normal and tumorigenic cells.
- Author:
In Woo PARK
1
;
Sam Sun LEE
;
Min Suk HEO
;
Soon Chul CHOI
Author Information
1. Department of Oral and Maxillofacial Radiology, College of Dentistry, Kangnung National University, Korea.
- Publication Type:Original Article
- Keywords:
irradiation;
cell viability;
apoptosis;
MTT assay;
TUNEL assay
- MeSH:
Apoptosis*;
Cell Line;
Cell Survival*;
DNA;
Humans;
In Situ Nick-End Labeling;
KB Cells;
Radiation Dosage*
- From:Journal of Korean Academy of Oral and Maxillofacial Radiology
1999;29(2):435-450
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: The study was aimed to detect the differences in the cell viability and the apoptosis induction after irradiation on normal and tumorigenic cells. MATERIALS AND METHODS: The study, that was generated for two human normal cells(RHEK, HGF-1) and two human tumor cells(KB, HT-1080), was tested using MTT assay at 1 day and 3 day after irradiation and TUNEL assay under confocal laser scanning microscope at 1 day after irradiation. Single irradiation of 0.5, 1, 2, 4, and 8 Gy were applied to the cells. The two fractions of 1, 2, 4, and 8 Gy were separated with a 4 hour time interval. The irradiation was done with 5.38 Gy/min dose rate using Cs-137 irradiator at room temperature. RESULTS AND CONCLUSIONS: 1. In 3-day group, the cell viability of HGF-1 cell was significantly decreased at 2, 4 and 8 Gy irradiation, the cell viability of KB cell was significantly decreased at 8 Gy irradiation and the cell viability of HT-1080 cell was significantly decreased at 4 and 8 Gy irradiation. 2. There was significant difference between RHEK and KB cell line in the cell viability of 3-day group at 8 Gy irradiation. There was significant difference between RHEK and HGF-1 cell line in the cell viability of 3-day group at 4 and 8 Gy irradiation. 3. There was a significantly decreased cell viability in 3-day group than those in 1-day group at 2, 4 and 8 Gy on HGF-1 cell, at 4 and 8 Gy on HT-1080 cell, at 8 Gy on KB cell. 4. We could detect DNA fragmented cells only on KB cell. Number of apoptotic cells of KB cell was significantly increased at 4 and 8 Gy irradiation. However, there was no correlation between cell viability and apoptosis. 5. On all 4 cell lines, there were no differences between single and split irradiation method in cell viability and apoptosis.
