FOXP3+T Cells and TGF-beta1 in Colonic Mucosa of Children with Crohn's Disease.
10.5223/kjpgn.2011.14.3.258
- Author:
Joo Hyun GIL
1
;
Jung Eun OH
;
Jeong Wan SEO
;
Min Sun CHO
;
Ky Young CHO
;
Eun Sun YOO
Author Information
1. Department of Pediatrics, Ewha Womans University School of Medicine, Seoul, Korea. jwseo@ewha.ac.kr
- Publication Type:Original Article
- Keywords:
T-lymphocytes;
Regulatory;
Crohn's disease;
FOXP3;
CD4;
Transforming growth factor beta;
Child;
Disease activity
- MeSH:
Biopsy;
Child;
Colon;
Crohn Disease;
Down-Regulation;
Hematocrit;
Humans;
Immunohistochemistry;
Mucous Membrane;
T-Lymphocytes;
T-Lymphocytes, Regulatory;
Transforming Growth Factor beta;
Transforming Growth Factor beta1
- From:Korean Journal of Pediatric Gastroenterology and Nutrition
2011;14(3):258-268
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: Forkhead box protein 3 (FOXP3)+T cells are the major regulatory T cells controlling all aspects of the immune response. Transforming growth factor-beta (TGF-beta) is a suppressive cytokine which mediates the suppressive action of FOXP3+T cells. The aim of this study was to investigate the role of FOXP3+T cells, TGF-beta in colonic mucosa of children with Crohn's disease (CD). METHODS: Colonic mucosal biopsies were obtained from 10 children with CD (12~15 years of age) and 11 control (8~15 years of age). Frequencies of FOXP3+T, CD4+T cells and TGF-beta1 expression were examined in the lamina propria (LP) and lymphoid aggregates or follicles (LA/F) by immunohistochemistry, and later evaluated by association with disease activity. RESULTS: In the LP of CD group, frequencies of FOXP3+T, CD4+T cells, proportion of FOXP3/CD4+T cells and TGF-beta1 expression significantly increased compared to the control. In the LA/F of CD group, frequency of FOXP3+T cells, proportion of FOXP3/CD4+T cells and TGF-beta1 expression significantly increased compared to the control (p<0.05). CD4+T cells also increased compared to the control, but this finding was not significant. In the LP and LA/F of CD group, frequency of FOXP3+T cells exhibited positive correlation with CD4+T cells (p<0.05). In the LP and LA/F of CD group, TGF-beta1 expression had positive correlation with CRP, Pediatric Crohn's Disease Activity Index, and negative correlation with hematocrit and albumin (p<0.05). CONCLUSION: Increased frequency of FOXP3+T cells and TGF-beta1 expression in colonic mucosa of CD can be interpreted as a compensatory increase towards achieving down-regulation of immune responses.
