Experimental Osteoporosis in Tail Suspended Rat.
- Author:
Ewy Ryong CHUNG
1
;
Jae Myeung CHUN
;
Jae Suk CHANG
;
Hyun Chul SHON
;
Jung Hwa KIM
;
Sang Won LEE
Author Information
1. Department of Orthopaedic Surgery, Asan Medical Center, College of Medicine, Univeristy of Ulsan, Seoul, Korea. jmchun@www.amc.seoul.kr
- Publication Type:Original Article
- Keywords:
Osteoporosis;
Skeletal unloading;
Osteoclast;
Receptor Activator of Nuclear Factor- kappaB Ligand(RANK-L);
Osteoprotegerin
- MeSH:
Animals;
Bone Density;
Bone Resorption;
Female;
Femur;
Hindlimb Suspension;
Humans;
Metabolism;
Osteoclasts;
Osteoporosis*;
Osteoprotegerin;
Rats*;
Rats, Sprague-Dawley;
RNA, Messenger;
Tail*
- From:Journal of Korean Orthopaedic Research Society
2003;6(1):35-46
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: To clarify whether the bone resorption in femur measured by the expression of OPG & RANK-L was increased in tail suspended rat. MATERIALS AND METHODS: Four-week-old female Sprague Dawley rats were divided into two groups. The experimental group (n=79) was housed and fed with 2 weeks of tail suspension, and reloaded for 8 weeks without tail suspension. The control group (n=46) was housed and fed for 10 weeks without tail suspension. Bone mineral densities, serum levels of ALP and TRAP were measured in both groups. The expressions of the mRNAs of OPG and RANK-L were analyzed by RT-PCR. RESULTS: The ALP and TRAP were increased in the experimental group during both tail suspension and reloading, which reflected the increased bone metabolism in the experimental group. In femur of the experimental group, the expression of the mRNA of RANK-L was increased during tail suspension, and the expression the mRNA of OPG was decreased. With reloading, the expression of the mRNA of RANK-L in femur was decreased, while the expression of the mRNA of OPG was increased.
