Effect of Epigallocatechin-3-Gallate on PMA-Induced MUC5B Expression in Human Airway Epithelial Cells.
- Author:
Eun Jung SONG
1
;
Chang Hoon BAE
;
Jong Yeon KIM
;
Yong Woon KIM
;
So Young PARK
;
Si Youn SONG
;
Yong Dae KIM
Author Information
1. Department of Physiology, Yeungnam University College of Medicine, Daegu, Korea.
- Publication Type:Original Article
- Keywords:
Epigallocatechin-3-gallate;
MUC5B;
p38;
Matrix metalloproteinase-9;
Airway epithelial cell
- MeSH:
Catechin;
Cholesterol;
Epithelial Cells*;
Gelatin;
Humans*;
Imidazoles;
Immunoenzyme Techniques;
Matrix Metalloproteinase 9;
p38 Mitogen-Activated Protein Kinases;
Phorbols;
Phosphorylation;
Protein Kinases;
Pyridines;
Real-Time Polymerase Chain Reaction;
Tea
- From:Clinical and Experimental Otorhinolaryngology
2013;6(4):237-242
- CountryRepublic of Korea
- Language:English
-
Abstract:
OBJECTIVES: Among the inflammatory mediators, phorbol 12-myristate 13-acetate (PMA) is associated with the regulation of MUC5B expression in the airway epithelial cells. Epigallocatechin-3-gallate (EGCG) is the major component of green tea extract. The biological activity of EGCG includes reduction of cholesterol and antioxidant activity, as well as anti-inflammatory effect. However, the precise action mechanism of anti-inflammatory effect of EGCG in the airway epithelial cells has not been fully defined. This study investigates the effect and the brief signaling pathway of EGCG on PMA-induced MUC5B expression in the airway epithelial cells. METHODS: In NCI-H292 airway epithelial cells, the effect and signaling pathway of EGCG on MUC5B expression were investigated using real-time polymerase chain reaction analysis, enzyme immunoassay, immunohistochemical analysis, gelatin zymography assay, and immunoblot analysis. RESULTS: In NCI-H292 airway epithelial cells, PMA induced MUC5B expression, phosphorylation of p38 mitogen-activated protein kinase (MAPK), and matrix metalloproteinase-9 (MMP-9) expression and protein activity. EGCG significantly decreased PMA-induced MUC5B expression, phosphorylation of p38 MAPK, and MMP-9 expression and protein activity. SB203580 (p38 MAPK inhibitor) significantly decreased PMA-induced MMP-9 expression. In addition, SB203580 and MMP-9 I (MMP-9 inhibitor) significantly decreased PMA-induced MUC5B expression. CONCLUSION: These results suggest that EGCG down-regulates PMA-induced MUC5B expression through the p38 MAPK dependent MMP-9 signaling pathway in human airway epithelial cells.
