Epigallocatechin-3-gallate Suppresses Galactose-alpha1,4-galactose-beta1,4-glucose Ceramide Expression in TNF-alpha Stimulated Human Intestinal Epithelial Cells Through Inhibition of MAPKs and NF-kappa B.
10.3346/jkms.2005.20.4.548
- Author:
Dong Oh MOON
1
;
Se Rim CHOI
;
Chang Min LEE
;
Gi Young KIM
;
Hee Jeong LEE
;
Yeong Min PARK
Author Information
1. Department of Microbiology and Immunology, and Medical Research Institute, and Laboratory of Dendritic Differentiation and Regulation, Pusan National University College of Medicine, Busan, Korea. immunpym@pusan.ac.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
epigallocatechin gallate;
Mitogen-Activated Protein Kinases;
NF-kappa B;
globotriaosylceramide;
Enterocytes;
Intestinal Mucosa
- MeSH:
Apoptosis/drug effects;
Blotting, Western;
Catechin/*analogs & derivatives/pharmacology;
Cell Nucleus/drug effects/metabolism;
Cell Proliferation/drug effects;
Dose-Response Relationship, Drug;
Epithelial Cells/drug effects/metabolism/pathology;
Flow Cytometry;
Galactosyltransferases/genetics;
Gene Expression Regulation, Enzymologic/drug effects;
Glucosyltransferases/genetics;
HT29 Cells;
Humans;
Intestinal Mucosa/drug effects/metabolism/pathology;
Mitogen-Activated Protein Kinases/antagonists & inhibitors/*metabolism;
NF-kappa B/antagonists & inhibitors/*metabolism;
Phosphorylation/drug effects;
Protein Transport/drug effects;
RNA, Messenger/genetics/metabolism;
Research Support, Non-U.S. Gov't;
Reverse Transcriptase Polymerase Chain Reaction;
Trihexosylceramides/*biosynthesis;
Tumor Necrosis Factor-alpha/*pharmacology
- From:Journal of Korean Medical Science
2005;20(4):548-554
- CountryRepublic of Korea
- Language:English
-
Abstract:
Intestinal epithelial cells (IECs) have been known to produce galactose-alpha1,4-galactose-beta1,4-glucose ceramide (Gb3) that play an important role in the mucosal immune response. The regulation of Gb3 is important to prevent tissue damage causing shiga like toxin. Epigallocatechin-3-gallate (EGCG) has been studied as anti-carcinogenic, anti-oxidant, anti-angiogenic, and anti-viral activities, and anti-diabetic. However, little is known between the expressions of Gb3 on IECs. The aim of this study was to examine the inhibitory effect of EGCG, a major ingredient of green tea, on Gb3 production via mitogen-activated protein kinases (MAPKs) and nuclear factor-kappa B (NF-kappa B) in the TNF-alpha stimulated human colon epithelial cells, HT29. To investigate how Gb3 is regulated, ceramide glucosyltransferase (CGT), lactosylceramide synthase (GalT2), and Gb3 synthase (GalT6) were analyzed by RT-PCR in HT 29 cells exposed to TNF-alpha in the presence or absence of EGCG. EGCG dose-dependently manner, inhibits TNF-alpha induced Gb3 expression by blocking in both the MAPKs and NF-kappaB pathways in HT29 cells. TNF-alpha enhanced CGT, GalT2 and GalT6 mRNA levels and EGCG suppressed the level of these enzymes enhanced by TNF-alpha treatment.
