Murine Model of Buckwheat Allergy by Intragastric Sensitization with Fresh Buckwheat Flour Extract.
10.3346/jkms.2005.20.4.566
- Author:
Soo Young LEE
1
;
Sejo OH
;
Kisun LEE
;
Young Ju JANG
;
Myung Hyun SOHN
;
Kyoung En LEE
;
Kyu Earn KIM
Author Information
1. Department of Pediatrics, Ajou University School of Medicine, Suwon, Korea. jsjs87@ajou.ac.kr
- Publication Type:Original Article ; Comparative Study ; Research Support, Non-U.S. Gov't
- Keywords:
Buckwheat;
Disease Models;
Animal;
Food Hypersensitivity
- MeSH:
Anaphylaxis/blood/immunology;
Animals;
Cell Proliferation/drug effects;
Comparative Study;
Disease Models, Animal;
Dose-Response Relationship, Drug;
Enzyme-Linked Immunosorbent Assay;
Fagopyrum/*immunology;
Female;
*Flour;
Food Hypersensitivity/blood/*immunology;
Immunoglobulin E/blood/immunology;
Immunoglobulin G/blood/immunology;
Interferon Type II/biosynthesis;
Interleukin-4/biosynthesis;
Interleukin-5/biosynthesis;
Mice;
Mice, Inbred C3H;
Plant Extracts/administration & dosage/immunology;
Research Support, Non-U.S. Gov't;
Spleen/cytology/drug effects/metabolism;
Stomach/drug effects/*immunology;
T-Lymphocytes/cytology/drug effects/metabolism;
Time Factors
- From:Journal of Korean Medical Science
2005;20(4):566-572
- CountryRepublic of Korea
- Language:English
-
Abstract:
Food allergies affect about 4% of the Korean population, and buckwheat allergy is one of the most severe food allergies in Korea. The purpose of the present study was to develop a murine model of IgE-mediated buckwheat hypersensitivity induced by intragastric sensitization. Young female C3H/HeJ mice were sensitized and challenged intragastricly with fresh buckwheat flour (1, 5, 25 mg/dose of proteins) mixed in cholera toxin, followed by intragastric challenge. Anaphylactic reactions, antigen-specific antibodies, splenocytes proliferation assays and cytokine productions were evaluated. Oral buckwheat challenges of sensitized mice provoked anaphylactic reactions such as severe scratch, perioral/periorbital swellings, or decreased activity. Reactions were associated with elevated levels of buckwheatspecific IgE antibodies. Splenocytes from buckwheat allergic mice exhibited significantly greater proliferative responses to buckwheat than non-allergic mice. Buckwheat-stimulated IL-4, IL-5, and INF-gamma productions were associated with elevated levels of buckwheat-specific IgE in sensitized mice. In this model, 1 mg and 5 mg dose of sensitization produced almost the same degree of Th2-directed immune response, however, a 25 mg dose showed blunted antibody responses. In conclusion, we developed IgE-mediated buckwheat allergy by intragastric sensitization and challenge, and this model could provide a good tool for future studies.
