Expression and Modulation of LL-37 in Normal Human Keratinocytes, HaCaT cells, and Inflammatory Skin Diseases.
10.3346/jkms.2005.20.4.649
- Author:
Ji Eun KIM
1
;
Beom Joon KIM
;
Mi Sook JEONG
;
Seong Jun SEO
;
Myeung Nam KIM
;
Chang Kwun HONG
;
Byung In RO
Author Information
1. Department of Dermatology, College of Medicine, Chung Ang University, Korea. drseo@hanafos.com
- Publication Type:Original Article ; Comparative Study ; Research Support, Non-U.S. Gov't
- Keywords:
CAP18 lipopolysaccharide-binding protein;
LL-37;
Keratinocytes;
HaCaT Cell;
Psoriasis;
Dermatitis;
Atopic
- MeSH:
Antimicrobial Cationic Peptides/analysis/*genetics;
Blotting, Western;
Cell Line;
Cells, Cultured;
Comparative Study;
Defensins/analysis/genetics;
Dose-Response Relationship, Drug;
Gene Expression/drug effects/radiation effects;
Humans;
Immunohistochemistry;
Keratinocytes/cytology/*metabolism;
Lipopolysaccharides/pharmacology;
Male;
RNA, Messenger/genetics/metabolism;
Research Support, Non-U.S. Gov't;
Reverse Transcriptase Polymerase Chain Reaction;
Skin/cytology/metabolism;
Skin Diseases/*genetics/metabolism/pathology
- From:Journal of Korean Medical Science
2005;20(4):649-654
- CountryRepublic of Korea
- Language:English
-
Abstract:
Defensins and cathelicidins (LL-37) are major antimicrobial peptides (AMPs) of the innate immune system of the human skin. In normal non-inflamed skin these peptides are negligible, but their expression can be markedly increased in inflammatory skin disease such as psoriasis. We designed this study to identify the expressions of LL-37 in normal human keratinocyte (NHK) and HaCaT cells after exposure to stimulants and to investigate difference of LL-37 expression accompanied with cell differentiation status, and come to understand difference of susceptibility to infection in atopic dermatitis and psoriasis. Expressions of LL-37 in NHKs and HaCaT cells were evaluated by using RT-PCR, Western blotting, and immunohistochemical (IHC) staining at 6, 12, and 24 hr post stimulation after exposure to Ultraviolet B irradiation and lipopolysaccharide. And expression of LL-37 in skin biopsy specimens from patients with atopic dermatitis and psoriasis was determined by immunohistochemical analysis. In time-sequential analyses of LL-37 expression revealed that LL-37 was expressed in NHKs, but not in HaCaT cells. IHC analysis confirmed the presence of abundant LL-37 in the epidermis of psoriasis. Therefore we deduced that expression of LL-37 is affected by UV irradiation, bacterial infection, and status of cell differentiation.
