A Case of Emetic Toxin Producing Bacillus cereusStrains Isolated from Outbreak.
10.5145/KJCM.2009.12.1.48
- Author:
Jong Hyun KIM
1
;
Eun Gyoung LIM
;
Hyun Chul JANG
;
Ju Young PARK
;
Sun Jin LEE
;
Mi Sun PARK
;
Gil Bae CHOI
;
Bok Kwon LEE
Author Information
1. Division of Enteric Bacterial Infections, Center for Infectious Diseases, National Institute of Health, Seoul, Korea. bokrates@nih.go.kr
- Publication Type:Case Report
- Keywords:
Emetic toxin;
Cereulide;
B. cereus
- MeSH:
Bacillus;
Bacillus cereus;
Coloring Agents;
Depsipeptides;
Diarrhea;
Eating;
Electrophoresis, Gel, Pulsed-Field;
Enterotoxins;
Enzyme-Linked Immunosorbent Assay;
Humans;
Polymerase Chain Reaction;
Republic of Korea;
Vacuoles;
Vomiting
- From:Korean Journal of Clinical Microbiology
2009;12(1):48-52
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Bacillus cereus causes two types of gastrointestinal diseases: emesis and diarrhea. It produces one emetic toxin and nine different enterotoxins. In March 2008, eight of a family became sick after eating slices of raw fish. We isolated emetic toxin producing B. cereus from the stools of 6 patients and 2 subclincal humans. In this study, the presence of enterotoxin genes, such as those of haemolysin BL (Hbl), nonhemolytic enterotoxin (Nhe), B. cereus enterotoxin T (BceT), enterotoxin FM (EntFM), cytotoxin K (cytK) and cereulide were assayed by polymerase chain reaction (PCR) methods. Their enterotoxin activities were assayed using the BCET- RPLA, Tecra ELISA kit and Hep-2 vacuole activity. Bacterial isolates were subtyped by pulsed-field gel electrophoresis (PFGE). This study demonstrates the emetic toxin-producing stains of B. cereus in clinical specimens, for the first time in the Republic of Korea.
