Changes of characterization of Salmonella Typhimurium isolate following sequential exposures to porcine neutrophil.
- Author:
Hee Soo LEE
1
;
Aeran KIM
;
Min YOUN
;
Ji Youn LEE
;
Suk Kyung LIM
;
Ho Young KANG
;
Han Sang YOO
;
Jung Won PARK
;
Sung Hwan WEE
;
Suk Chan JUNG
Author Information
1. Animal, Plant and Fisheries Quarantine and Inspection Agency, Anyang 430-016, Korea. leehs0415@korea.kr
- Publication Type:Original Article
- Keywords:
live vaccine;
neutrophil;
plasmid curing;
Salmonella Typhimurium
- MeSH:
Animals;
Humans;
Injections, Intraperitoneal;
Lethal Dose 50;
Mice;
Neutrophils;
Parents;
Plasmids;
Salmonella;
Salmonella typhimurium;
Sprains and Strains;
Trehalose;
Xylose
- From:Korean Journal of Veterinary Research
2013;53(1):29-35
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
To develop a live vaccine candidate using an attenuated strain of Salmonella Typhimurium (ST), biochemical properties, plasmid profile, PFGE patterns and pathogenic analysis of the ST isolate were carried out after sequential passage of the ST isolate in porcine neutrophils. By the passage, the ability of the neutrophil-adapted isolate to utilize d-xylose was lost, while the ability of the strain to ferment trehalose was delayed after 2 or more days of the culture. Also, changes including deletion of the gene fragments were observed in PFGE analysis of the neutrophil-adapted isolates. Two plasmids, 105kb and 50kb, were cured in the strain passaged over 15 times in porcine neutrophils. The 50% of lethal dose (LD50) of the parent strain was changed from 1 x 10(5) LD50 to 6 x 10(6) LD50 by the passage in intraperitoneal injection of the strains into mice. These results suggested that bacterial genotypic and phenotypic responses might be globally altered depending on the inside environment of neutrophils.
