A Simple, Reproducible, Inexpensive, Yet Old-Fashioned Method for Determining Phagocytic and Bactericidal Activities of Macrophages.
10.3349/ymj.2016.57.2.283
- Author:
Masakazu KANEKO
1
;
Yoshiko EMOTO
;
Masashi EMOTO
Author Information
1. Laboratory of Immunology, Department of Laboratory Sciences, Gunma University Graduate School of Health Sciences, Maebashi, Japan. masashi.emoto@gmail.com
- Publication Type:Research Support, Non-U.S. Gov't ; Review
- Keywords:
Anti-bacterial agent;
gentamicin;
macrophage;
phagocytosis
- MeSH:
Anti-Bacterial Agents/*pharmacology;
Gentamicins/*pharmacology;
Macrophages/*drug effects;
Phagocytosis/*drug effects
- From:Yonsei Medical Journal
2016;57(2):283-290
- CountryRepublic of Korea
- Language:English
-
Abstract:
Macrophages (Mphi) play a pivotal role in the protection system by recognizing and eliminating invading pathogenic bacteria. Phagocytosis and the killing of invading bacteria are major effector functions of Mphi. Although the phagocytic and bactericidal activities of Mphi have been analyzed via several methods using a light microscope, a fluorescence microscope, or a fluorescence-activated cell sorter, expensive materials and equipment are usually required, and the methods are rather complicated. Moreover, it is impossible to determine both the phagocytic and bactericidal activities of Mphi simultaneously using these methods. In this review, we describe a simple, reproducible, inexpensive, yet old-fashioned method (antibiotic protection assay) for determining the phagocytic and bactericidal activities of Mphi.
