Swiprosin-1 Expression Is Up-Regulated through Protein Kinase C-theta and NF-kappaB Pathway in T Cells.
- Author:
Young Dae KIM
1
;
Min Sung KWON
;
Bo Ra NA
;
Hye Ran KIM
;
Hyun Su LEE
;
Chang Duk JUN
Author Information
- Publication Type:Original Article
- Keywords: T cells; Swiprosin-1; Protein kinase C; Actin remodeling
- MeSH: Actins; Biology; Calcimycin; Down-Regulation; Gene Expression; Humans; Ionomycin; Lymphocytes; NF-kappa B; Precursor Cells, B-Lymphoid; Protein Kinase C; Protein Kinases; RNA, Small Interfering; T-Lymphocytes
- From:Immune Network 2013;13(2):55-62
- CountryRepublic of Korea
- Language:English
- Abstract: Swiprosin-1 exhibits the highest expression in CD8+ T cells and immature B cells and has been proposed to play a role in lymphocyte biology through actin remodeling. However, regulation of swiprosin-1 gene expression is poorly understood. Here we report that swiprosin-1 is up-regulated in T cells by PKC pathway. Targeted inhibition of the specific protein kinase C (PKC) isotypes by siRNA revealed that PKC-theta is involved in the expression of swiprosin-1 in the human T cells. In contrast, down-regulation of swiprosin-1 by A23187 or ionomycin suggests that calcium-signaling plays a negative role. Interestingly, swiprosin-1 expression is only reduced by treatment with NF-kappaB inhibitors but not by NF-AT inhibitor, suggesting that the NF-kappaB pathway is critical for regulation of swiprosin-1 expression. Collectively, these results suggest that swiprosin-1 is a PKC-theta-inducible gene and that it may modulate the late phase of T cell activation after antigen challenge.
