Detection of Respiratory Viruses in Children by Multiplex Reverse Transcriptase PCR, Direct Immunofluorescence Assay, and Shell Vial Culture.
10.5145/KJCM.2009.12.3.110
- Author:
Kui Hyun YOON
1
;
Ji Hyun CHO
Author Information
1. Department of Laboratory Medicine, Wonkwang University Sanbon Hospital, Gunpo, Korea.
- Publication Type:Original Article
- Keywords:
Direct immunofluorescence assay;
Shell vial culture;
Multiplex PCR;
Respiratroy virus
- MeSH:
Adenoviridae;
Child;
Fluorescent Antibody Technique;
Fluorescent Antibody Technique, Direct;
Humans;
Multiplex Polymerase Chain Reaction;
Orthomyxoviridae;
Paramyxoviridae Infections;
Respiratory Syncytial Viruses;
Reverse Transcriptase Polymerase Chain Reaction;
RNA-Directed DNA Polymerase;
Viruses
- From:Korean Journal of Clinical Microbiology
2009;12(3):110-115
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Direct immunofluorescence assay (DFA) and shell vial culture (SVC) have been used to diagnose respiratory viral infections. Recently a multiplex reverse transcriptase PCR (mRT-PCR) for 12 respiratory viruses has been introduced. We evaluated the diagnostic usefulness of these methods. METHODS: Among 275 nasopharyngeal aspirates (NPAs) received from pediatric patients during the 3-month period from May through July, 2007, 122 samples were selected so as to include diverse viruses and varying numbers of DFA-positive cells for mRT-PCR. Also, the results of the 85 NPAs that had been analyzed by both DFA and SVC were reviewed retrospectively. RESULTS: Detection rates for the seven major respiratory viruses, respiratory syncytial virus (RSV), influenza virus A and B, parainfluenza virus 1, 2, and 3, and adenovirus by DFA vs mRT-PCR were 32.0% and 55.7%, and by DFA vs SVC were 32.9% and 40.0%. A number of adenovirus detected by DFA vs mRT-PCR were 12 and 22, and by DFA vs SVC were 6 and 18. A number of RSV detected were 3 and 6, and 13 and 8, respectively. CONCLUSIONS: mRT-PCR detected the respiratory viruses at the highest rate, followed by SVC and DFA in a decreasing order. However, DFA and multiplex PCR were more sensitive than SVC for RSV, while SVC was more sensitive than the other methods for adenovirus.
