The Protective Effect of Chlorpromazine on Flurothyl-Induced Seizure.
- Author:
Dong Wook KIM
1
Author Information
1. Department of Pediatrics, Ilsan Paik Hospital, College of Medicine, Inje University, Goyang, Korea. dwkim@ilsanpaik.ac.kr
- Publication Type:Original Article
- Keywords:
Chlorpromazine;
Glutamate dehydrogenase;
Flurothyl;
Seizure susceptibility;
Rat
- MeSH:
Amination;
Animals;
Chlorpromazine*;
Flurothyl;
Glutamate Dehydrogenase;
Glutamic Acid;
Hindlimb;
Hope;
Humans;
Injections, Subcutaneous;
Male;
Neurotransmitter Agents;
Posture;
Rats;
Rats, Sprague-Dawley;
Seizures*
- From:
Journal of the Korean Child Neurology Society
2003;11(2):234-237
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: Chlorpromazine(CPZ) is known to inhibit glutamate ehydrogenase(GDH). Reductive amination of alpha-ketoglutarate is catalyzed by GDH and forms glutamate, a major excitatory neurotransmitter. Thus, I hypothesized that CPZ might have a seizure- protective effect by inhibiting glutamate release from the excitatory presynaptic nerve terminal. The present study was designed to investigate the protective effect of CPZ on flurothyl-induced seizure in rats. METHODS: Twenty-eight male Sprague-Dawley rats were equally divided into 2 groups. CPZ(20 mg/kg) was administered to experimental animals by subcutaneous injection, while normal saline to control animals. Twenty minutes later, seizures were chemically induced by flurothyl infusion(40 microL/min). Seizure susceptibility was defined as the latency from the start of flurothyl infusion to the onset of a generalized seizure(loss of posture with bilateral hindlimb tonic extension). Shorter latency reflects greater seizure susceptibility. RESULTS: The mean(+/-SEM) seizure latency in the experimental group was 539.2 (+/-17.5) seconds, and it was significantly longer than 432.4(+/-21.9) seconds in the control group(P<0.001). CONCLUSION: This study demonstrates that CPZ decrease flurothyl-induced seizure susceptibility in rats. This result suggests that CPZ may have a seizure-protective effect. I hope that further studies on this issue should be performed in a near future.
