Development of a Tissue-Engineered Vascular Graft Using Autologous Bone Marrow-Derived Cells and Biodegradable Polymer Scaffold.
- Author:
Seung Woo CHO
1
;
Dong Ik KIM
;
Hee Jung PARK
;
Sang Hyun LIM
;
Oju JEON
;
Soo Hyun KIM
;
Young Ha KIM
;
Cha Yong CHOI
;
Min Jae LEE
;
Jong Sung KIM
;
In Sung JANG
;
Byung Soo KIM
Author Information
1. Department of Chemical Engineering, Hanyang University, Seoul, Korea. dikim@smc.samsung.co.kr
- Publication Type:In Vitro ; Original Article
- Keywords:
Tissue-engineered vascular graft;
Bone marrow-derived cell;
Biodegradable polymer scaffold
- MeSH:
Actins;
Arteries;
Blood Vessel Prosthesis*;
Bone Marrow;
Cell Proliferation;
Collagen;
Extracellular Matrix Proteins;
Fungi;
Models, Animal;
Muscle, Smooth;
Myosin Heavy Chains;
Polyglycolic Acid;
Polymers*;
Rupture;
Thrombosis;
Transplants
- From:Journal of the Korean Society for Vascular Surgery
2003;19(2):124-131
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: The objective of this study is to develop a tissue-engineered vascular graft using autologous bone marrow-derived cells (BMCs) and biodegradable polymer scaffold. METHOD: Autologous canine BMCs were isolated from bone marrow aspirate and cultured. A tubular scaffold was fabricated by immersing polyglycolic acid (PGA) sheet in poly (glycolide-co-caprolactone) (PGCL) solution and wrapping it around a cylindrical mold. The expanded BMCs were seeded onto the PGA/PGCL tubular scaffold (internal diameter: 7 mm, length: 35 mm) and further cultured in vitro for 1 week. The graft was anastomosed to the abdominal artery in a canine model. One week after implantation, the retrieved graft was investigated by histological and immunohistochemical analyses. RESULT: Cultured BMCs differentiated into endothelial-like and smooth muscle-like cells. The PGA tubular scaffold reinforced with PGCL was successfully implanted in an animal model without graft rupture. The vascular graft engineered with BMCs was occluded at 1 week after implantation due to thrombus formation. Histological and immunohistochemical analyses of the retrieved graft revealed that extracellular matrix proteins such as smooth muscle alpha-actin, smooth muscle myosin heavy chain and collagen were produced partially in the graft media. CoNCLUSION: The tissue-engineered vascular graft developed in this study led to graft failure due to early occlusion. Nevertheless, it is confirmed that the PGA/PGCL scaffold has microstructures appropriate for cell proliferation and good mechanical properties. This result suggests the possibile application of this scaffold as a material for engineering of diseased vascular tissues.
