The Effect of Lipo-PGE1 on Ischemia-Reperfusion Injury and Endothelin-1 Concentrations After Canine Partial Liver Transplantation.
- Author:
Ki Youl SEO
1
;
Il Hwan MOON
;
Kwon YOO
;
Hye Kyung JUNG
;
Ku Yong CHUNG
;
Kum Ja CHOI
Author Information
1. Department of Internal Medicine, Ewha Womans University Hospital, Seoul, Korea. kiyoulseo@yahoo.co.kr
- Publication Type:Original Article
- Keywords:
Transplantation/Liver/Partial liver transplantation;
Ischemia-reperfusion injury;
Lipo-PGE1;
Endothelin-1
- MeSH:
Alprostadil*;
Animals;
Catheterization;
Dogs;
Endothelin-1*;
Humans;
Liver Transplantation*;
Liver*;
Pulmonary Circulation;
Reperfusion;
Reperfusion Injury*;
Tissue Donors
- From:The Korean Journal of Hepatology
2001;7(4):475-484
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND/AIM: The lipo-PGE1, known for being more stable during pulmonary circulation and having more targeting effect, has been reported to inhibit ET-1 induced stellate cell contraction. We assessed the effect of lipo-PGE1 on the change of ET-1 concentration and the relationship between ET-1 concentration and the liver damage. METHODS: Mongrel dogs weighing about 25 kg were divided into a control (n=6) and a lipo-PGE1 (n=6) group. Partial liver allotransplantation was performed. In the lipo-PGE1 group, lipo-PGE1 was slowly infused through splenic venous cannulation during the donor liver harvesting procedure (50 microgram) and continuously infused (60 microgram/day) for 48 hours after reperfusion. The AST, ALP, LDH and ET-1 concentrations were monitored RESULTS: The AST and ALP levels of the lipo-PGE1 group were significantly lower than those of the control group both at 1 hour and 48 hours after reperfusion. The LDH level in the lipo-PGE1 group was lower at 1 hour and 48 hours after reperfusion. But there was no statistical difference between the two groups. The baseline ET-1 concentration of the lipo-PGE1 group was eight times higher than that of the control group. The ET-1 concentration was elevated gradually in the control group. There was no significant difference between the two groups at 48 hours. There was no correlation between ET-1 concentrations and AST, ALP, LDH levels. CONCLUSION: This study demonstrated the hepatoprotective effect of the lipo-PGE1 against ischemia-reperfusion injury in canine partial liver allotransplantation. However, the baseline ET-1 level was eight times higher in the lipo-PGE1 group than that of the control group in spite of the hepatoprotective effects of the lipo-PGE1.
