IgG Western Blot for Confirmatory Diagnosis of Equivocal Cases of Toxoplasmosis by EIA-IgG and Fluorescent Antibody Test.
10.3347/kjp.2013.51.4.485
- Author:
Imen KHAMMARI
1
;
Fatma SAGHROUNI
;
Alia YAACOUB
;
Sondoss GAIED MEKSI
;
Hinda ACH
;
Lamia GARMA
;
Akila FATHALLAH
;
Moncef BEN SAID
Author Information
1. UR 12 SP 34 Research Unit, Ministry of Public Health, Tunis, Tunisia. imenkhammari@yahoo.fr
- Publication Type:Brief Communication ; Validation Studies
- Keywords:
Toxoplasma gondii;
toxoplasmosis;
pregnant women;
serodiagnosis;
immunoblot
- MeSH:
Adult;
Antibodies, Protozoan/*analysis/blood;
Blotting, Western/*methods;
Female;
Fluorescent Antibody Technique/*methods;
Humans;
Immunoenzyme Techniques/*methods;
Immunoglobulin M/*analysis/blood;
Pregnancy;
Pregnancy Complications, Parasitic/blood/*diagnosis;
Toxoplasmosis/blood/*diagnosis;
Young Adult
- From:The Korean Journal of Parasitology
2013;51(4):485-488
- CountryRepublic of Korea
- Language:English
-
Abstract:
The performance values of available techniques used in serodiagnosis of toxoplasmosis are satisfactory but they raise problems of equivocal and discordant results for very low IgG titers. Recently marketed, LDBio-Toxo II IgG Western blot (IB) showed an excellent correlation with the dye test. We estimated the proportion of equivocal and discordant results between the enzyme immunoassay Platelia Toxo IgG (EIA-IgG) and fluorescent antibody test (FAT) and assessed the usefulness of the IB as a confirmatory test. Out of 2,136 sera collected from pregnant women, 1,644 (77.0%) tested unequivocally positive and 407 (19.0%) were negative in both EIA-IgG and FAT. The remaining 85 (4%) sera showed equivocal or discordant results. Among them, 73 (85.9%) were positive and 12 (14.1%) were negative in IB. Forty-one (89.1%) equivocal sera in EIA-IgG and 46 (86.8%) equivocal sera in FAT were positive in IB. Reducing the cut-off values of both screening techniques improved significantly their sensitivity in detecting very low IgG titers at the expense of their specificity. In conclusion, equivocal results in routine-used techniques and their discordance in determination of the immune status in pregnancy women were not uncommon. IB test appeard to be highly useful in these situations as a confirmatory technique.
