Dirofilaria repens in Vietnam: Detection of 10 Eye and Subcutaneous Tissue Infection Cases Identified by Morphology and Molecular Methods.
10.3347/kjp.2012.50.2.137
- Author:
Nguyen Van DE
1
;
Thanh Hoa LE
;
Jong Yil CHAI
Author Information
1. Department of Parasitology, Hanoi Medical University, Hanoi, Vietnam. ngvdeyhn@gmail.com
- Publication Type:Case Reports ; Research Support, Non-U.S. Gov't
- Keywords:
Dirofilaria repens;
case report;
eye;
subcutaneous tissue;
PCR;
mitochondrial cox1;
Vietnam
- MeSH:
Adult;
Aged;
Animals;
Cyclooxygenase 1/genetics;
Dirofilaria repens/anatomy & histology/genetics/*isolation & purification;
Dirofilariasis/*diagnosis/parasitology/*pathology;
Eye/*parasitology;
Eye Diseases/parasitology;
Female;
Humans;
Male;
Middle Aged;
Molecular Diagnostic Techniques/methods;
Parasitology/methods;
Phylogeny;
Polymerase Chain Reaction;
Sequence Analysis, DNA;
Sequence Homology;
Skin Diseases/parasitology;
Subcutaneous Tissue/*parasitology;
Vietnam
- From:The Korean Journal of Parasitology
2012;50(2):137-141
- CountryRepublic of Korea
- Language:English
-
Abstract:
From 2006 to 2010, hospitals in Hanoi treated 10 human patients for dirofilariasis. The worms were collected from parasitic places, and identification of the species was completed by morphology and molecular methods. Ten parasites were recovered either from the conjunctiva (n=9) or subcutaneous tissue (n=1). The parasites were 4.0-12.5 cm in length and 0.5-0.6 mm in width. Morphological observations suggested all parasites as Dirofilaria repens. Three of the 10 parasites (1 from subcutaneous tissue and 2 from eyes) were used for molecular confirmation of the species identification. A portion of the mitochondrial cox1 (461 bp) was amplified and sequenced. Nucleotide and amino acid homologies were 95% and 99-100%, respectively, when compared with D. repens (Italian origin, GenBank AJ271614; DQ358814). This is the first report of eye dirofilariasis and the second report of subcutaneous tissue dirofilariasis due to D. repens in Vietnam.
