Expression and Characterization of alpha-Methylacyl CoA Racemase from Anisakis simplex Larvae.
10.3347/kjp.2012.50.2.165
- Author:
Bong Jin KIM
1
;
Sun Mi KIM
;
Min Kyung CHO
;
Hak Sun YU
;
Yong Seok LEE
;
Hee Jae CHA
;
Meesun OCK
Author Information
1. Department of Internal Medicine, Kosin University College of Medicine, Busan 602-703, Korea.
- Publication Type:Brief Communication ; Research Support, Non-U.S. Gov't
- Keywords:
Anisakis simplex L3;
cDNA library;
immunoscreening;
excretory-secretory product;
alpha-methylacyl CoA racemase
- MeSH:
Amino Acid Sequence;
Animals;
Anisakis/*enzymology/genetics;
Cloning, Molecular;
Cluster Analysis;
Gene Expression Profiling;
Gene Library;
Humans;
Immunohistochemistry;
Larva/enzymology/genetics;
Mice;
Mice, Inbred ICR;
Molecular Sequence Data;
Phylogeny;
Rabbits;
Racemases and Epimerases/genetics/*metabolism;
Sequence Homology, Amino Acid
- From:The Korean Journal of Parasitology
2012;50(2):165-171
- CountryRepublic of Korea
- Language:English
-
Abstract:
Larval excretory-secretory products of Anisakis simplex are known to cause allergic reactions in humans. A cDNA library of A. simplex 3rd-stage larvae (L3) was immunoscreened with polyclonal rabbit serum raised against A. simplex L3 excretory-secretory products to identify an antigen that elicits the immune response. One cDNA clone, designated as alpha-methylacyl CoA racemase (Amacr) contained a 1,412 bp cDNA transcript with a single open reading frame that encoded 418 amino acids. A. simplex Amacr showed a high degree of homology compared to Amacr orthologs from other species. Amacr mRNA was highly and constitutively expressed regardless of temperature (10-40degrees C) and time (24-48 hr). Immunohistochemical analysis revealed that Amacr was expressed mainly in the ventriculus of A. simplex larvae. The Amacr protein produced in large quantities from the ventriculus is probably responsible for many functions in the development and growth of A. simplex larvae.
