Size Control of 99mTc-tin Colloid Using PVP and Buffer Solution for Sentinel Lymph Node Detection.
10.3346/jkms.2015.30.6.816
- Author:
Eun Mi KIM
1
;
Seok Tae LIM
;
Myung Hee SOHN
;
Hwan Jeong JEONG
Author Information
1. Department of Nuclear Medicine, Molecular Imaging & Therapeutic Medicine Research Center, Cyclotron Research Center, Institute for Medical Science, Biomedical Research Institute, Chonbuk National University Medical School and Hospital, Jeonju, Korea. jayj
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Sentinel Lymph Node;
Lymphoscintigraphy;
99mTc-tin Colloid;
Polyvinylpyrrolidone (PVP)
- MeSH:
Animals;
Buffers;
Cell Line, Tumor;
Humans;
Lymph Nodes/*radionuclide imaging;
Lymphatic Metastasis;
Metal Nanoparticles/chemistry/ultrastructure;
Mice;
Neoplasms, Experimental/*radionuclide imaging;
Particle Size;
Povidone/*chemistry;
Rabbits;
Radiopharmaceuticals/*chemical synthesis;
Reproducibility of Results;
Sensitivity and Specificity;
Technetium Compounds/*chemistry;
Tin/*chemistry;
Tin Compounds/*chemistry
- From:Journal of Korean Medical Science
2015;30(6):816-822
- CountryRepublic of Korea
- Language:English
-
Abstract:
Colloidal particle size is an important characteristic that allows mapping sentinel nodes in lymphoscintigraphy. This investigation aimed to introduce different ways of making a 99mTc-tin colloid with a size of tens of nanometers. All agents, tin fluoride, sodium fluoride, poloxamer-188, and polyvinylpyrrolidone (PVP), were mixed and labeled with 99mTc. Either phosphate or sodium bicarbonate buffers were used to adjust the pH levels. When the buffers were added, the size of the colloids increased. However, as the PVP continued to increase, the size of the colloids was controlled to within tens of nanometers. In all samples, phosphate buffer added PVP (30 mg) stabilized tin colloid (99mTc-PPTC-30) and sodium bicarbonate solution added PVP (50 mg) stabilized tin colloid (99mTc-BPTC-50) were chosen for in vitro and in vivo studies. 99mTc-BPTC-50 (<20 nm) was primarily located in bone marrow and was then secreted through the kidneys, and 99mTc-PPTC-30 (>100 nm) mainly accumulated in the liver. When a rabbit was given a toe injection, the node uptake of 99mTc-PPTC-30 decreased over time, while 99mTc-BPTC-50 increased. Therefore, 99mTc-BPTC-50 could be a good candidate radiopharmaceutical for sentinel node detection. The significance of this study is that nano-sized tin colloid can be made very easily and quickly by PVP.
