Relative risk of virulence factors in Candida-infected mouse.
- Author:
Donghwa KIM
1
;
Woon Seob SHIN
;
Kyoung Ho LEE
;
Kyunghoon KIM
;
Yoon Sun PARK
;
Joo Young PARK
;
Choon Myung KOH
Author Information
1. Department of Microbiology, Yonsei University Wonju College of Medicine, Wonju, Kangwon-Do, 220-701, South Korea. kdtan@wonju.yonsei.ac.kr
- MeSH:
Animals;
Antibodies;
Candida;
Candida albicans;
Candidiasis;
Diagnosis;
Epithelial Cells;
Epithelium;
Humans;
Hydrophobic and Hydrophilic Interactions;
Hyphae;
Immunization, Passive;
Immunocompromised Host;
Mice*;
Peptide Hydrolases;
Phospholipases;
Prevalence;
Risk Factors;
Survival Rate;
Virulence Factors*;
Virulence*
- From:Journal of the Korean Society for Microbiology
2000;35(4):317-324
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Candida albicans is one of the most frequently isolated fungal pathogens in human. Recently, the prevalence of candida infection has markedly increased, partially due to the increase of immunocompromised hosts. Proposed virulence factors of the pathogenic Candida are the ability to form hyphae to adhere to epithelial cell surfaces, and to secrete acid proteinases and phospholipases. We measured the relative cell surface hydrophobicity (CSH) and the ability of proteinase production (PROT), phospholipase production (PLase), adherence to host epithelium (ADH), and hyphal transition (Germ). The relative risk of virulence factors was analyzed by lethality test in murine model of hematogeneously disseminated candidal infection. According to Cox's proportional hazard analysis, the statistically significant virulence factors were PROT, ADH, and CSH. PROT was the highest risk factor of them. To evaluate the applicability for the diagnosis and treatment of Candidiasis, we examined the protective effect of the active and passive immunizations with the materials purified from virulence factors and antibodies to them in Candia-infected mice model. The mean survival times of active and passive immunized groups were slightly longer than those of non-immunized groups.
