Simultaneous Determination of 11 Marker Compounds in Gumiganghwal-tang by HPLC-DAD and LC-MS.
10.20307/nps.2016.22.4.238
- Author:
Jin Bae WEON
1
;
Youn Sik JUNG
;
Gahee RYU
;
Woo Seung YANG
;
Choong Je MA
Author Information
1. Department of Medical Biomaterials Engineering, College of Biomedical Science, Kangwon National University, Chuncheon 200-701, Korea. cjma@kangwon.ac.kr
- Publication Type:Original Article
- Keywords:
Simultaneous determination;
Gumiganghwal-tang;
HPLC-DAD;
LC-MS
- MeSH:
Calibration;
Chromatography, High Pressure Liquid;
Common Cold;
Eugenol;
Glycyrrhizic Acid;
Limit of Detection;
Linear Models;
Methods;
Quality Control;
Spectrometry, Mass, Electrospray Ionization;
Trifluoroacetic Acid
- From:Natural Product Sciences
2016;22(4):238-245
- CountryRepublic of Korea
- Language:English
-
Abstract:
Gumiganghwal-tang has been used for the treatment of common cold for a long-time. We developed an accurate and sensitive high performance liquid chromatography-diode array detection (HPLC-DAD) and electrospray ionization mass spectrometry method for the simultaneous determination of ferulic acid, baicalin, bergapten, methyl eugenol, glycyrrhizin, oxypeucedanin, wogonin, nodakenin, atractylenolide III, imperatorin, and atractylenolide I in Gumiganghwal-tang samples. The analytes were separated on a Shiseido C18 column (5 µm, 4.6 mm I.D. × 250 mm) with gradient elution with acetonitrile and 0.1% trifluoroacetic acid. Eleven compounds were quantitatively determined by HPLC-DAD and identified by LC-MS data. We also validated this method. The calibration curves of all the compounds showed good linear regression. The limits of detection and the limits of quantification ranged from 0.04 to 0.63 and from 0.12 to 1.92 µg/mL, respectively. The relative standard deviation values of intra- and inter-days of this method represented less than 2.9%. The recoveries were found to be in the range of 90.06 – 107.66%. The developed method has been successfully applied to the analysis of Gumiganghwal-tang samples. The established HPLC method could be used to quality control of Gumiganghwal-tang.
