The Preventive and Curative Effect of Cyanidin-3β-D-Glycoside and Its Metabolite Protocatechuic Acid Against TNBS-induced Colitis in Mice.
10.20307/nps.2016.22.4.282
- Author:
Se Eun JANG
1
;
Jong Ryul CHOI
;
Myung Joo HAN
;
Dong Hyun KIM
Author Information
1. Department of Food and Nutrition, Kyung Hee University, 26, Kyungheedae-ro, Dongdaemun-gu, Seoul 02447, Korea. mjhan@khu.ac.kr
- Publication Type:Original Article
- Keywords:
Colitis;
Cyanidin-3β-D-glycoside;
Procatechuic acid
- MeSH:
Animals;
Biotransformation;
Colitis*;
Colon;
Cyclooxygenase 2;
Functional Food;
Gastrointestinal Microbiome;
Interleukin-6;
Macrophages, Peritoneal;
Mice*;
Peroxidase
- From:Natural Product Sciences
2016;22(4):282-286
- CountryRepublic of Korea
- Language:English
-
Abstract:
Cyanidin-3β-D-glycoside (C3G), which is widely distributed in herbal medicines and functional foods, exhibits anti-inflammatory, anti-oxidant, and anti-scratching behavioral effects. Orally administered C3G is metabolized to protocatechuic acid (PA) by gut microbiota. Therefore, we compared the anti-colitic effect of C3G to that of PA in mice with 2,4,6-trinitrobenzenesulfonic acid (TNBS)-induced colitis. Orally administered C3G and PA preventively and curatively ameliorated TNBS-induced colitis parameters, including macroscopic colitis score, colon shortening, and increase of myeloperoxidase activity. Treatment with C3G or PA also inhibited the expression of cyclooxygenase-2, inducible NO synthatase, IL-1β, IL-6, and TNF-α and the activation of NF-κB in the colon of mice with TNBS-induced colitis. Furthermore, these also inhibited lipopolysaccharide-induced NF-κB activation and TNF-α expression in peritoneal macrophages. The anti-colitic effect of PA was more effective than C3G. Orally administered PA more potently attenuate colitis than C3G by inhibiting NF-κB activation and the anti-colitic efficacy of C3G may be dependent on the biotransformation of C3G to PA by gut microbiota.
