Maximization of Extracted Condition of Pro-angiogenic Components in Citrus unshiu Peels using Dimethyl Sulfoxide.
10.20307/nps.2016.22.4.287
- Author:
Jungwhoi LEE
1
;
Myungseung KIM
;
Jae Hoon KIM
Author Information
1. College of Applied Life Science, SARI, Jeju National University, Jeju-do 690-756, Korea. sdjd1108@kaist.ac.kr kimjh@jejunu.ac.kr
- Publication Type:Original Article
- Keywords:
Angiogenesis;
Citrus unshiu peels;
Hesperidin;
Narirutin;
DMSO;
MeOH
- MeSH:
2-Propanol;
Citrus*;
Dimethyl Sulfoxide*;
Ethanol;
Hesperidin;
Human Umbilical Vein Endothelial Cells;
Methanol;
Phosphorylation;
Solvents
- From:Natural Product Sciences
2016;22(4):287-292
- CountryRepublic of Korea
- Language:English
-
Abstract:
Aqueous extraction of Citrus unshiu peels (AECUP) is mainly comprised with pro-angiogenichesperidin and narirutin. In this study, we report approaches to increasing the yields of extracted hesperidin and narirutinfrom Citrus unshiu peels using proper solvents. Significantly improved yields of both compounds were obtained using methanol and dimethyl sulfoxide (DMSO) compared to acetonitrile, ethyl acetate, ethanol, and isopropyl alcohol. Especially, effect of DMSO was by far the better of the two solvents in extraction of hesperidin. In addition, the DMSO extracted hesperidin significantly induced the pro-angiogenic effects of human umbilical vein endothelial cells (HUVECs) and markedly up-regulated phosphorylation of the ERK1/2 signaling pathway. These results demonstrate that pro-angiogenic inducer; hesperidin and narirutin can be simply, easily, and effectively extracted from Citrus unshiu peels.
