Transgenic expression of Korean type hepatitis C virus core protein and related mutants in mice.
- Author:
Ai Guo WANG
1
;
Hyung Bae MOON
;
Young Ho LEE
;
Seong Lan YU
;
Hyun Jung KWON
;
Ying Hao HAN
;
Wan FANG
;
Tae Hoon LEE
;
Kyung Lib JANG
;
Sang Keun KIM
;
Dae Yeul YU
;
Dong Seok LEE
Author Information
1. Laboratory of Human Genomics Korea Research Institute of Bioscience and Biotechnology (KRIBB) Daejeon 305-806, Korea. lee10@kribb.re.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
core;
dysplasia;
hepatitis C virus;
transgenic mice
- MeSH:
Amino Acid Sequence;
Animals;
Base Sequence;
Gene Expression;
Genetic Vectors/genetics;
Hepatitis C/*pathology/virology;
Hepatitis, Viral, Animal/*pathology/virology;
Hepatocytes/pathology/virology;
Liver/pathology/*virology;
Mice;
Mice, Transgenic;
Molecular Sequence Data;
Mutation/genetics;
RNA, Messenger/chemistry/metabolism;
Research Support, Non-U.S. Gov't;
Transgenes;
Viral Core Proteins/analysis/*genetics/metabolism
- From:Experimental & Molecular Medicine
2004;36(6):588-592
- CountryRepublic of Korea
- Language:English
-
Abstract:
Hepatitis C virus (HCV) is a major causative agent in liver disease. In order to investigate if Korean type HCV core protein and its related mutants, S99Q and S116I, are cytopathic to liver, three types of transgenic mice were established. The expression of transgenes was confirmed by HCV specific RT-PCR and Western immunoblotting. The livers of all wild type core and S116I transgenic lineages remained largely histologically normal. However, the livers of the S99Q transgenic mice showed significant high level of cell dysplasia associated with the transgene expression in hepatocytes largely located around the central veins by in situ hybridization analysis. In conclusion, the mutant HCV core protein at S99Q may contribute to the progress of HCV induced liver disease.
