Differential expression of Matrix Metalloproteinase (MMP)-2, -9 in normal and severe preeclamptic human placentas.
- Author:
Yeo Jung MOON
1
;
Han Sung HWANG
;
Young Han KIM
;
Ja Young KWON
;
Yong Won PARK
Author Information
1. Department of Obstetrics and Gynecology, Yonsei University Health System, Seoul, Korea. yhkim522@yuhs.ac
- Publication Type:Original Article
- Keywords:
MMP-2;
MMP-9;
Severe preeclampsia;
Placenta
- MeSH:
Blotting, Western;
Cesarean Section;
Female;
Gene Expression;
Humans;
Placenta;
Pre-Eclampsia;
Pregnancy;
Proteins;
Trophoblasts
- From:Korean Journal of Obstetrics and Gynecology
2008;51(11):1245-1253
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVE: The aim of the study was to investigate the differential expression of Matrix Metalloproteinase (MMP) -2,-9 in the normal and severe preeclamptic placenta. METHODS: Placentas were obtained from women undergoing cesarean section with normal (n=10) and severe preeclamptic (n=10) pregnancies. Semi-quantitative RT-PCR was done to detect the placental gene expression of MMP-2,-9. Western blot analysis was performed to identify MMP-2,-9 protein expression in each placenta. Immunohistochemical staining were employed to localize MMP-2,-9 in placental tissues. RESULTS: MMP-2,-9 genes were expressed in both normal and severe preeclamptic placenta. There were lower expressions MMP-2,-9 in severe preeclamptic placentas than in normal. MMP-2,-9 proteins were all present in each placental tissue. The expression for MMP-2,-9 was weaker in severe preeclamptic placenta than in normal. MMP-2,-9 were localized only to the trophoblast, and were also weakly positive in severe preeclamptic placenta compared with normal. CONCLUSIONS: MMP-2,-9 expressions were decreased in severe preeclampsia placenta compared to those from normal placenta. This study suggests that decreased expression of MMP-2,-9 may have a role in the development of severe preeclampsia.
