Immunohistochemical localization of galectin-3 in the brain with Theiler's murine encephalomyelitis virus (DA strain) infection.
- Author:
Taekyun SHIN
1
;
Francisco J CARRILLO-SALINAS
;
Ana FELIU MARTINEZ
;
Miriam MECHA
;
Carmen GUAZA
Author Information
1. Department of Veterinary Anatomy, College of Veterinary Medicine, Jeju National University, Jeju 690-756, Korea. shint@jejunu.ac.kr
- Publication Type:Original Article
- Keywords:
encephalomyelitis;
galectin-3;
macrophages;
microglia;
Theiler's murine encephalomyelitis virus
- MeSH:
Animals;
Apoptosis;
Astrocytes;
Brain*;
Cell Movement;
Demyelinating Diseases;
Encephalomyelitis;
Galectin 3*;
Immunohistochemistry;
Inflammation;
Macrophages;
Mice;
Microglia;
Neurons;
Oligodendroglia;
Theilovirus*;
Viruses
- From:Korean Journal of Veterinary Research
2013;53(3):159-162
- CountryRepublic of Korea
- Language:English
-
Abstract:
Galectin-3 is a beta-galactoside-binding lectin that plays a role in neuroinflammation through cell migration, proliferation, and apoptosis. In the present study, regulation of galectin-3 was examined in the brain of mice infected with the Daniel strain of Theiler's murine encephalomyelitis virus (TMEV) at days 7 and 81 post-infection by immunohistochemistry. Immunohistochemistry revealed that galectin-3 was mainly localized in ionized calcium-binding adapter 1-positive macrophages/activated microglia, but not in Iba-1-positive ramified microglia. Galectin-3 was also weakly detected in some astrocytes in the same encephalitic lesions, but not in neurons and oligodendrocytes. Collectively, the present findings suggest that galectin-3, mainly produced by activated microglia/macrophages, may be involved in the pathogenesis of virus induced acute inflammation in the early stage as well as the chronic demyelinating lesions in Daniel strain of TMEV induced demyelination model.
