Osteocalcin Expression and Mineralization in Developing Tooth of Xenopus laevis.
10.11620/IJOB.2015.40.1.001
- Author:
Jung Hoe PARK
1
;
Ki tak KWON
;
Byung Keon PARK
;
Young Hoon LEE
Author Information
1. Department of Oral Anatomy, School of Dentistry, and Institute of Oral Biosciences, Chonbuk National University, Korea. yhlee@chonbuk.ac.kr, bkpark@chonbuk.ac.kr
- Publication Type:Original Article
- Keywords:
osteocalcin;
von Kossa;
mineralization;
tooth development;
Xenopus
- MeSH:
Animals;
Calcium;
Dental Papilla;
Enamel Organ;
Extracellular Matrix;
Humans;
In Situ Hybridization;
Mice;
Odontoblasts;
Odontogenesis;
Osteocalcin*;
Osteogenesis;
Rats;
RNA, Messenger;
Tooth Germ;
Tooth*;
Xenopus;
Xenopus laevis*
- From:International Journal of Oral Biology
2015;40(1):1-9
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Osteocalcin (OC) is the most abundant noncollagenous protein of extracellular matrix in the bone. In an OC deficient mouse, bone formation rates are increased in cancellous and cortical bones. OC is known as a negative regulator of mineral apposition. OC is also expressed in the tooth of the rat, bovine, and human. However, little is known about OC during tooth development in Xenopus. The purpose of this study is to compare the expression of OC with mineralization in the developing tooth of Xenopus, by using von Kossa staining and in situ hybridization. At stage 56, the developmental stage of tooth germ corresponds to the cap stage, and an acellular zone was apparent between the dental papilla and the enamel organ. From stage 57, calcium deposition was revealed by von Kossa staining prior to OC expression, and the differentiated odontoblasts forming predentin were located at adjoining predentin. At stage 58, OC transcripts were detected in the differentiated odontoblasts. At stage 66, OC mRNA was expressed in the odontoblasts, which was aligned in a single layer at the periphery of the pulp. These findings suggest that OC may play a role in mineralization and odontogenesis of tooth development in Xenopus.
