Molecular identification of Mycoplasma cynos from laboratory beagle dogs with respiratory disease.
- Author:
Sunhwa HONG
1
;
Okjin KIM
Author Information
- Publication Type:Letter
- Keywords: Mycoplasma; PCR; consensus; Mycoplasma cynos; Beagle; dog
- MeSH: Animals; Animals, Laboratory; Autopsy; Cats; Consensus; DNA; Dogs; Felis; Hyperplasia; Lung; Molar; Mycoplasma; Pneumonia, Mycoplasma; Polymerase Chain Reaction
- From:Laboratory Animal Research 2012;28(1):61-66
- CountryRepublic of Korea
- Language:English
- Abstract: In this study, we examined a colony of 20 beagle dogs in a laboratory animal facility. Mycoplasma was detected by consensus PCR assay in 1 dog with respiratory and constitutional symptoms. None of the other dogs were affected. The dog was euthanized and necropsied. In postmortem examinations, gray or plum-colored gross lesions were found on the lung, most commonly in the apical and cardiac lobes. Some lesions showed clear demarcation and consolidation. Microscopic examination showed peribronchiolar lymphoid hyperplasia and interstitial thickening, lesions pathognomonic for mycoplasma pneumonia. To identify canine Mycoplasma species, we used species-specific PCR reactions for M. arginini, M. canis, M. cynos, M. edwardii, M. felis, M. gateae, M. maculosum, M. molare, M. opalescens, M. spumans, Mycoplasma sp. HRC 689, and M. collis. As the result, we identified Mycoplasma cynos by amplification of DNA extracted from lung tissue of the laboratory beagle dog with respiratory disease.
