Diagnosis of canine brucellosis using recombinant ribosomal protein L7/L12.
- Author:
Hyang Keun LEE
1
;
Jong Wan KIM
;
Yun Mi HA
;
Moon HER
;
Ji Yeon KIM
;
Kichan LEE
;
Sung Il KANG
;
Suk Chan JUNG
Author Information
1. Animal, Plant and Fisheries Quarantine and Inspection Agency, Anyang 430-824, Korea. herm@korea.kr
- Publication Type:Original Article
- Keywords:
Brucella canis;
indirect-ELISA;
ribosomal protein L7/L12
- MeSH:
Aborted Fetus;
Brucella;
Brucella canis;
Brucellosis;
Clone Cells;
Enzyme-Linked Immunosorbent Assay;
Humans;
Pets;
Placenta;
Ribosomal Proteins;
Sensitivity and Specificity;
Veterinarians
- From:Korean Journal of Veterinary Research
2012;52(1):25-31
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Brucella (B.) canis is mainly transmitted by direct or indirect contact with aborted fetuses and placenta. It's also known to be able to infect human, which likely results in providing veterinarians and companion animal owners for infectious risk. To develop diagnostic ELISA, we cloned and expressed rp1L gene of B. canis, which encodes the ribosomal protein L7/L12. Using this purified recombinant protein, indirect-ELISA (iELISA) was evaluated using 78 positive and 44 negative sera. The sensitivity and the specificity of iELISA were 94% and 89%, respectively. The results indicated that indirect-ELISA using recombinant ribosomal protein L7/L12 may be useful for diagnosis of canine brucellosis.
