An improved multiplex PCR for diagnosis and differentiation of Mycoplasma hyopneumoniae and Mycoplasma hyorhinis.
- Author:
Abhijit K BARATE
1
;
Hwi Young LEE
;
Hye Won JEONG
;
Lam Quang TRUONG
;
Hong Gu JOO
;
Tae Wook HAHN
Author Information
1. Department of Veterinary Medicine, College of Veterinary Medicine & Institute of Veterinary Science, Kangwon National University, Chuncheon 200-701, Korea. twhahn@kangwon.ac.kr
- Publication Type:Original Article
- Keywords:
enzootic swine pneumonia;
multiplex PCR;
Mycoplasma
- MeSH:
DNA;
Imidazoles;
Korea;
Lung;
Multiplex Polymerase Chain Reaction;
Mycoplasma;
Mycoplasma hyopneumoniae;
Mycoplasma hyorhinis;
Nitro Compounds;
Swine
- From:Korean Journal of Veterinary Research
2012;52(1):39-43
- CountryRepublic of Korea
- Language:English
-
Abstract:
A multiplex PCR was developed for the simultaneous detection and differentiation of Mycoplasma (M.) hyopneumoniae and M. hyorhinis in clinical samples. Improved sensitivity is advantage of this technique over the previously reported multiplex assay. It was capable of detecting as little as 125 fg genomic DNA from M. hyopneumoniae and 62.5 fg genomic DNA from M. hyorhinis. Application of this multiplex PCR method to field isolates showed that M. hyopneumoniae and M. hyorhinis were present in 29% (107 of 370) of lung specimens and no mycoplasmas were detected in 56% (208 of 370) of the slaughtered pigs' lungs. At the farm level, M. hyopneumoniae and M. hyorhinis were detected in 34 of 36 (94.4%) randomly selected farms. We conclude that this assay would prove itself a value tool for monitoring these mycoplasmal infections and both M. hyopneumoniae and M. hyorhinis have been widely spread in swine herds of Korea.
