Leu72Met408 Polymorphism of the Ghrelin Gene Is Associated With Early Phase of Gastric Emptying in the Patients With Functional Dyspepsia in Japan.
- Author:
Hiroshi YAMAWAKI
1
;
Seiji FUTAGAMI
;
Mayumi SHIMPUKU
;
Tomotaka SHINDO
;
Yuuta MARUKI
;
Hiroyuki NAGOYA
;
Yasuhiro KODAKA
;
Hitomi SATO
;
Katya GUDIS
;
Tetsuro KAWAGOE
;
Choitsu SAKAMOTO
Author Information
- Publication Type:Original Article
- Keywords: Depression; Gastric emptying; Ghrelin; Polymorphism, genetic
- MeSH: Breath Tests; Classification; Depression; DNA; Dyspepsia*; Gastric Emptying*; Genotype; Ghrelin*; GTP-Binding Proteins; Healthy Volunteers; Humans; Japan*; Polymerase Chain Reaction; Polymorphism, Genetic; Polymorphism, Single Nucleotide; Surveys and Questionnaires
- From:Journal of Neurogastroenterology and Motility 2015;21(1):93-102
- CountryRepublic of Korea
- Language:English
- Abstract: BACKGROUND/AIMS: There are no available data about the relationship between ghrelin gene genotypes and early phase of gastric emptying in functional dyspepsia (FD) as defined by Rome III classification. METHODS: We enrolled 74 patients presenting with typical symptoms of FD and 64 healthy volunteers. Gastric motility was evaluated using the 13C-acetate breath test. We used Rome III criteria to evaluate upper abdominal symptoms and self-rating questionnaires for depression (SRQ-D) scores to determine status of depression. The Arg51Gln (346G>A), preproghrelin (3056T>C), Leu72Met (408C>A), Gln90Leu (3412T>A) and G-protein beta3 (825C>T) polymorphisms were analyzed in the DNA from blood samples of enrolled subjects. Genotyping was performed by polymerase chain reaction. RESULTS: There was a significant relationship between the Gln90Leu3412 genotype and SRQ-D score in FD patients (P = 0.009). Area under the curve at 15 minutes (AUC15) value was significantly associated with the Leu72Met408 genotype (P = 0.015) but not with entire gastric emptying. CONCLUSIONS: The Leu72Met (408C>A) single nucleotide polymorphism was significantly associated with early phase of gastric emptying in FD patients. Further studies will be necessary to clarify the association between ghrelin gene single nucleotide polymorphisms and early phase of gastric emptying in FD patients.
