Evolution of Genetic Polymorphisms of Plasmodium falciparum Merozoite Surface Protein (PfMSP) in Thailand.
10.3347/kjp.2014.52.1.105
- Author:
Jiraporn KUESAP
1
;
Wanna CHAIJAROENKUL
;
Kanchanok KETPRATHUM
;
Puntanat TATTIYAPONG
;
Kesara NA-BANGCHANG
Author Information
1. Faculty of Allied Health Sciences, Thammasat University, Pathumthani, 12121, Thailand.
- Publication Type:Brief Communication ; Research Support, Non-U.S. Gov't
- Keywords:
Plasmodium falciparum;
merozoite surface protien;
genetic polymorphism;
Thailand
- MeSH:
Antigens, Protozoan/*genetics;
DNA, Protozoan/genetics;
Evolution, Molecular;
Humans;
Malaria, Falciparum/parasitology;
Merozoite Surface Protein 1/*genetics;
Plasmodium falciparum/*classification/*genetics/isolation & purification;
Polymerase Chain Reaction;
*Polymorphism, Genetic;
Protozoan Proteins/*genetics;
Thailand
- From:The Korean Journal of Parasitology
2014;52(1):105-109
- CountryRepublic of Korea
- Language:English
-
Abstract:
Plasmodium falciparum malaria is a major public health problem in Thailand due to the emergence of multidrug resistance. The understanding of genetic diversity of malaria parasites is essential for developing effective drugs and vaccines. The genetic diversity of the merozoite surface protein-1 (PfMSP-1) and merozoite surface protein-2 (PfMSP-2) genes was investigated in a total of 145 P. falciparum isolates collected from Mae Sot District, Tak Province, Thailand during 3 different periods (1997-1999, 2005-2007, and 2009-2010). Analysis of genetic polymorphisms was performed to track the evolution of genetic change of P. falciparum using PCR. Both individual genes and their combination patterns showed marked genetic diversity during the 3 study periods. The results strongly support that P. falciparum isolates in Thailand are markedly diverse and patterns changed with time. These 2 polymorphic genes could be used as molecular markers to detect multiple clone infections and differentiate recrudescence from reinfection in P. falciparum isolates in Thailand.
