The Effects of Dopamin D-1 Agonist (SKF-38393) on Expression of Substance-P & Enkephalin mRNA in the Striatum of Hemiparkinsonian Rat Model.
- Author:
Seung Hwan LEE
1
;
Do Young KWON
;
Sung Wook YU
;
Seong Beom KOH
;
Min Kyu PARK
;
Hyun KIM
;
Kun Woo PARK
;
Dae Hie LEE
Author Information
1. Deparment of Neurology, College of Medicine, Korea University, Korea. kunu@korea.ac.kr
- Publication Type:Original Article
- Keywords:
Parkinson rat model;
Enkephalin;
Substance-P;
SKF-38393
- MeSH:
2,3,4,5-Tetrahydro-7,8-dihydroxy-1-phenyl-1H-3-benzazepine;
Animals;
Dopamine;
Dopamine Agonists;
Enkephalins*;
Models, Animal*;
Rats*;
RNA, Messenger*
- From:Journal of the Korean Neurological Association
2003;21(5):527-533
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: The selective D1 dopamine agonist has been known to play a stimulatory role in substance-P synthesis in the striatum, but its effect on the enkephalin mRNA expression has not been well known in the striatum of unilateral 6-hydroxydopamine(OHDA)-lesioned rat. So we investigated the effect of selective D1 dopaminergic agonist on substance-P, enkephalin mRNA expression in the striatum with different time interval. METHODS: The lesioned rats were divided into two groups (treated and non-treated). Each group was subdivided according to time course after lesioning (2nd, 4th and 8th week). Dopamine 1 receptor agonist, SKF-38393 (5 mg/kg/ip) and the same volume of saline was injected to treated and nontreated group respectively. The levels of enkephalin and substance-P mRNA were determined by in situ-hydridization and the expression of mRNA levels were compared between the groups. RESULTS: The expression of striatal enkephalin mRNA was increased on lesioned side in all groups. Especially, SKF-38393 enhanced the striatal expression of enkephalin mRNA after lesioning 2nd week. After lesioning 4th week and 8th week, the effect of SKF-38393 was not significant. The striatal expression of substance-P mRNAs was significantly decreased on the lesioned side, especially at the 2nd weeks. This decrement of substance-P mRNA was reversed by SKF-38393 at the 2nd week. CONCLUSIONS: These data show that the selective D1 agonist SKF-38393 have an agonistic effect on direct pathway but antagonistic effect on indirect pathway in early course of Parkinsonian rat model.
