groEL Gene Analysis of Borrelia afzelii Isolated in Korea.
- Author:
Hyo Soon PARK
1
;
Jung Hee LEE
;
Eun Ju JEONG
;
Won Jong JANG
;
Kyung Hee PARK
;
Bum Joon KIM
;
Yoon Hoh KOOK
;
Seung Hyun LEE
Author Information
1. Department of Microbiology, College of Medicine, Konkuk University, Chugju, Chungchungbuk-Do 380-701, Korea. shlee@kku.ac.kr
- Publication Type:Original Article
- Keywords:
groEL gene;
B. afzelii;
Heterogeneity
- MeSH:
Animals;
Borrelia burgdorferi Group*;
Borrelia*;
DNA;
Heat-Shock Proteins;
Ixodes;
Korea*;
Murinae;
Population Characteristics
- From:Journal of Bacteriology and Virology
2004;34(1):1-8
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Eleven Borrelia afzelii strains, isolated from Ixodes nipponensis and Apodemus agrarius in Korea, were characterized by groEL gene analysis. Results from previous studies suggested that the groEL gene, which encodes the 60-kDa heat shock protein GroEL, was useful for the differentiation of B. burgdorferi sensu lato. The B. afzelii isolates could be divided into two groups by the phylogenetic tree constructed by UPGMA method and Tsp509 I PCR-RFLP analysis. The result suggested that the groEL gene is useful for identification and characterization of B. burgdorferi sensu lato though a short DNA fragment (310 bp) of the gene was sequenced and compared each other, and that Korean B. afzelii strains are heterogeneous genotypically.
