Evaluation of antibacterial activity and osteoblast-like cell viability of TiN, ZrN and (Ti(1-x)Zr(x))N coating on titanium.
- Author:
Min Kyung JI
1
;
Sang Won PARK
;
Kwangmin LEE
;
In Chol KANG
;
Kwi Dug YUN
;
Hyun Seung KIM
;
Hyun Pil LIM
Author Information
- Publication Type:Original Article
- Keywords: Periimplantitis; Antibacterial activity; Titanium nitride (TiN); Zirconium nitride (ZrN); Streptococcus mutans; Porphyromonas gingivalis
- MeSH: Biofilms; Cell Survival*; Humans; Microscopy, Electron, Scanning; Peri-Implantitis; Porphyromonas gingivalis; RNA, Messenger; Stem Cells; Streptococcus mutans; Tin*; Titanium*; Zirconium
- From:The Journal of Advanced Prosthodontics 2015;7(2):166-171
- CountryRepublic of Korea
- Language:English
- Abstract: PURPOSE: The aim of this study was to evaluate antibacterial activity and osteoblast-like cell viability according to the ratio of titanium nitride and zirconium nitride coating on commercially pure titanium using an arc ion plating system. MATERIALS AND METHODS: Polished titanium surfaces were used as controls. Surface topography was observed by scanning electron microscopy, and surface roughness was measured using a two-dimensional contact stylus profilometer. Antibacterial activity was evaluated against Streptococcus mutans and Porphyromonas gingivalis with the colony-forming unit assay. Cell compatibility, mRNA expression, and morphology related to human osteoblast-like cells (MG-63) on the coated specimens were determined by the XTT assay and reverse transcriptase-polymerase chain reaction. RESULTS: The number of S. mutans colonies on the TiN, ZrN and (Ti(1-x)Zr(x))N coated surface decreased significantly compared to those on the non-coated titanium surface (P<0.05). CONCLUSION: The number of P. gingivalis colonies on all surfaces showed no significant differences. TiN, ZrN and (Ti(1-x)Zr(x))N coated titanium showed antibacterial activity against S. mutans related to initial biofilm formation but not P. gingivalis associated with advanced periimplantitis, and did not influence osteoblast-like cell viability.
