Effects of Oxidative Stress and Antioxidant on the Expression of Heme Oxygenase-1 in Human RPE.
10.3341/jkos.2009.50.8.1247
- Author:
Young Jun KIM
1
;
Hee Seung CHIN
Author Information
1. Department of Ophthalmology, Inha University School of Medicine, Incheon, Korea. hschin@inha.ac.kr
- Publication Type:Original Article
- Keywords:
Age related macular degeneration;
Antioxidant;
Heme oxygenase-1;
Oxidative stress;
Retinal pigment epithelium
- MeSH:
Antioxidants;
Heme;
Heme Oxygenase-1;
Humans;
Hydrogen Peroxide;
Macular Degeneration;
Oxidative Stress;
Retinal Diseases;
Retinal Pigment Epithelium;
Retinaldehyde;
RNA, Messenger
- From:Journal of the Korean Ophthalmological Society
2009;50(8):1247-1253
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: To evaluate the effects of oxidative stress and antioxidantson heme oxygenase-1 (HO-1) in cultured human retinal pigment epithelial (RPE) cells. METHODS: Cultured RPE cells were challenged with different concentrations of hydrogen peroxide (H2O2), and the HO-1 mRNA level was determined by RT-PCR after 24 hours and 48 hours of incubation independently. Additionally, the HO-1 mRNA level was measured after preincubating RPE cells with N-acetylcystein (NAC) as the antioxidant for 30 minutes and then challenging the cells with H2O2. RESULTS: The expression of the HO-1 mRNA level increased after H2O2 exposure, and this level was proportional to the increased H2O2 concentration (p<0.05). Expression of the HO-1 mRNA level decreased when the RPE cells were preincubated with NAC (p<0.05). CONCLUSIONS: In human RPE cells, the HO-1 level increased in proportion to the degree of oxidative stress and decreased with exposure to antioxidants. Expression of HO-1 may be helpful in preventing retinal disease, which occurs due to oxidative stresses such as age-related macular degeneration.
