Modified CHROMagar Acinetobacter Medium for Direct Detection of Multidrug-Resistant Acinetobacter Strains in Nasal and Rectal Swab Samples.
10.3343/alm.2013.33.3.193
- Author:
Wonkeun SONG
1
;
Jacob LEE
;
Taek Kyung KIM
;
Min Jeong PARK
;
Han Sung KIM
;
Jae Seok KIM
Author Information
1. Department of Laboratory Medicine, Hallym University College of Medicine, Chuncheon, Korea. swonkeun@hallym.or.kr
- Publication Type:Brief Communication ; Evaluation Studies
- Keywords:
Modified CHROMagar Acinetobacter;
Multidrug-resistant Acinetobacter spp.;
Nasal swab;
Rectal swab
- MeSH:
Acinetobacter/drug effects/*isolation & purification;
Acinetobacter Infections/diagnosis/microbiology;
Anti-Bacterial Agents/pharmacology;
*Drug Resistance, Multiple, Bacterial/drug effects;
Humans;
Intensive Care Units;
Microbial Sensitivity Tests;
Nose/*microbiology;
Reagent Kits, Diagnostic;
Rectum/*microbiology
- From:Annals of Laboratory Medicine
2013;33(3):193-195
- CountryRepublic of Korea
- Language:English
-
Abstract:
This study aimed to investigate whether CHROMagar Acinetobacter medium (CHROMagar, France) in combination with an antimicrobial supplement (modified CHROMagar Acinetobacter; CHROMagar, France) can be used for detecting and isolating multidrug-resistant Acinetobacter species (MRA) in nasal and rectal surveillance cultures. Nasal and rectal swab samples were collected from patients in an intensive care unit at a teaching hospital. The samples were used to inoculate modified CHROMagar Acinetobacter plates, which were examined after 24 and 48 hr of incubation at 37degrees C. Their susceptibility against the antimicrobial agents meropenem, imipenem, ciprofloxacin, and amikacin was analyzed using the Etest (bioMerieux, France). A total of 406 paired samples (406 nasal swabs and 406 rectal swabs) were obtained from 226 patients, and 120 samples (28 nasal and 28 rectal cultures, 47 nasal cultures only, and 17 rectal cultures only) yielded MRA. Seventy-five MRA isolates (18.5%) were recovered from the 406 nasal samples, and 45 MRA isolates (11.1%) were recovered from the 406 rectal samples. Of the 120 MRA isolates, 3 (2.5%) were detected only after 48 hr of incubation. The use of modified CHROMagar Acinetobacter together with nasal and rectal swabs and 1-day incubation is an effective surveillance tool for detecting MRA colonization.
