The Effect of Combined Immunosuppressive Treatment with FK 506-Cyclophosphamide on Surgical Angiogenesis.
- Author:
Yang Guk CHUNG
1
;
Yong Koo KANG
;
Yun Kyung LEE
;
Young Jun YANG
Author Information
1. Department of Orthopedic Surgery, College of Medicine, The Catholic University of Korea, Seoul, Korea. ygchung@catholic.ac.kr
- Publication Type:Original Article
- Keywords:
Non-vascularized bone xenograft;
Surgical angiogenesis;
FK 506- cyclophosphamide immunosuppression;
Capillary density
- MeSH:
Capillaries;
Heterografts;
Immunosuppression;
Silicone Elastomers;
Transplants
- From:Journal of Korean Orthopaedic Research Society
2006;9(1):58-64
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: To evaluate the effect of combined immunosuppressive treatment with FK 506-cyclophosphamide on surgical angiogenesis in non-vascularized bone xenografts. MATERIALS AND METHODS: Twenty-seven hamster-to-rat non-vascularized bone xenografts were performed. For surgical angiogenesis, recipient origin saphenous arteriovenous bundles were implanted into the medullary canals of graft bones. Immunosuppression with FK 506-cyclophosphamide was performed in group 1, not in group 2, control group. At postoperative 4 weeks, microangiography with microfil was conducted. Decalcified bones were cleared and the area of newly formed capillary, capillary density, were quantitatively evaluated using Scion image program. Bone specimens stained with hematoxylin-eosin were evaluated histologically also. RESULTS: There was no significant difference of capillary densities between immunosuppressed group and non-immunosuppressed control group (0.37+/-0.03/0.39+/-0.02, P=0.58). On histological examination, both groups showed neoangiogenesis into the medullary canals and endosteal bones. CONCLUSION: FK 506-cyclophosphamide immunosuppression did not suppress the angiogenesis in non-vascularized bone xenografts, FK 506-cyclophosphamide combination of immunosuppression will be useful even in the condition of surgical angiogenesis.
