The Effect of Anti-inflammatory Agents on the Permeability of Trabecular Meshwork Cell Monolayers.
10.3341/jkos.2017.58.10.1155
- Author:
Hye Ri KANG
1
;
Jung Hoon LEE
;
Jae Woo KIM
Author Information
1. Department of Ophthalmology, Catholic University of Daegu School of Medicine, Daegu, Korea. jwkim@cu.ac.kr
- Publication Type:Original Article
- Keywords:
Bromfenac;
Loteprednol;
Permeability;
Prednisolone;
Trabecular meshwork cells
- MeSH:
Anti-Inflammatory Agents*;
Humans;
Loteprednol Etabonate;
Permeability*;
Prednisolone;
Trabecular Meshwork*
- From:Journal of the Korean Ophthalmological Society
2017;58(10):1155-1159
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: To compare the effects of anti-inflammatory agents, specifically bromfenac, loteprednol, and prednisolone, on the permeability of cultured human trabecular meshwork cell (HTMC) monolayers. METHODS: HTMCs were cultured until confluency in the inner chamber of Transwell, then exposed to 1/1,000 or 1/500 diluted commercial 0.1% bromfenac, 0.5% loteprednol, and 1% prednisolone for 24 hours. The permeabilities of carboxyfluorescein through the HTMC monolayer were measured with a spectrofluorometer after 2 hours in the outer chamber. Cellular viabilities were assessed with an 3-[4,5–dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay. RESULTS: Bromfenac and loteprednol diluted at 1/1,000 or 1/500 did not significantly affect the cellular survival (p > 0.05). Bromfenac did not affect the permeability via the HTMC monolayer (p > 0.05) and loteprednol decreased the permeability (p < 0.05). In addition, 1/2,000 prednisolone also decreased the permeability (p < 0.05). CONCLUSIONS: Among the anti-inflammatory agents, the non-steroidal anti-inflammatory agent bromfenac did not affect the permeability, while loteprednol and prednisolone decreased the permeability through the HTMC monolayer. Thus, loteprednol and prednisolone may decrease the trabecular outflow.
