Activation of NF-kappa B in the cisplatin-induced apoptosis of oral squamous cell carcinoma.
- Author:
Jong Chun SEO
1
;
Iel Yong SUNG
;
Jong Roul KIM
Author Information
1. Department of Oral and Maxillofacial Surgery, College of Dentistry, Pusan National University, Korea. jorkim@pusan.ac.kr
- Publication Type:Original Article
- Keywords:
Apoptosis;
NF-kappa B;
Cisplatin
- MeSH:
Apoptosis*;
Carcinoma, Squamous Cell*;
Cell Line;
Cisplatin;
DNA Fragmentation;
Humans;
In Situ Nick-End Labeling;
Mouth Neoplasms;
Negotiating;
NF-kappa B*
- From:Journal of the Korean Association of Oral and Maxillofacial Surgeons
2006;32(2):94-100
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Purpose: This study was done to confirm the role of NF-kappa B in cisplatin-induced apoptosis of oral squamous cell carcinoma. Materials and Methods: Five cell lines originated from different oral cancer patients were tested for the apoptosis by the treatment of cisplatin. These cells showed different degree of cisplatin-resistance and the order is OSCC-2> OSCC-3> OSCC-5> OSCC-1> OSCC-4. OSCC-2 and OSCC-4 cells were assayed for the apoptosis by measuring DNA fragmentation and TUNEL staining after cisplatin treatment. While OSCC-4 cells showed apoptosis, OSCC-2 cells showed no or very slight apoptosis by cisplatin treatment. Next, It was determined whether NF-kappa B activation is required in mediating cisplatin-induced apoptosis of OSCC-4 . Result: The result was that elevated NF-kappa B activity mediated cisplatin-induced apoptosis. Conclusion: In conclusion, these findings suggest that NF-kappa B activation is essential to cisplatin-induced apoptosis and it may be involved in cisplatin resistance in OSCC cells.
